TY - JOUR
T1 - Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico
AU - Verástegui-Valdés, Myrthala M.
AU - Zhang, Yu Jing
AU - Rivera-Orduña, Flor N.
AU - Cheng, Hai Ping
AU - Sui, Xing Hua
AU - Wang, En Tao
N1 - Publisher Copyright:
© 2014 Elsevier GmbH.
PY - 2014
Y1 - 2014
N2 - In order to investigate bean-nodulating rhizobia in different types of soil, 41 nodule isolates from acid and alkaline soils in Mexico were characterized. Based upon the phylogenetic studies of 16S rRNA, atpD, glnII, recA, rpoB, gyrB, nifH and nodC genes, the isolates originating from acid soils were identified as the phaseoli symbiovar of the Rhizobium leguminosarum-like group and Rhizobium grahamii, whereas the isolates from alkaline soils were defined as Ensifer americanum sv. mediterranense and Rhizobium radiobacter. The isolates of ". R. leguminosarum" and E. americanum harbored nodC and nifH genes, but the symbiotic genes were not detected in the four isolates of the other two species. It was the first time that ". R. leguminosarum" and E. americanum have been reported as bean-nodulating bacteria in Mexico. The high similarity of symbiotic genes in the Rhizobium and Ensifer populations showed that these genes had the same origin and have diversified recently in different rhizobial species. Phenotypic characterization revealed that the ". R. leguminosarum" population was more adapted to the acid and low salinity conditions, while the E. americanum population preferred alkaline conditions. The findings of this study have improved the knowledge of the diversity, geographic distribution and evolution of bean-nodulating rhizobia in Mexico.
AB - In order to investigate bean-nodulating rhizobia in different types of soil, 41 nodule isolates from acid and alkaline soils in Mexico were characterized. Based upon the phylogenetic studies of 16S rRNA, atpD, glnII, recA, rpoB, gyrB, nifH and nodC genes, the isolates originating from acid soils were identified as the phaseoli symbiovar of the Rhizobium leguminosarum-like group and Rhizobium grahamii, whereas the isolates from alkaline soils were defined as Ensifer americanum sv. mediterranense and Rhizobium radiobacter. The isolates of ". R. leguminosarum" and E. americanum harbored nodC and nifH genes, but the symbiotic genes were not detected in the four isolates of the other two species. It was the first time that ". R. leguminosarum" and E. americanum have been reported as bean-nodulating bacteria in Mexico. The high similarity of symbiotic genes in the Rhizobium and Ensifer populations showed that these genes had the same origin and have diversified recently in different rhizobial species. Phenotypic characterization revealed that the ". R. leguminosarum" population was more adapted to the acid and low salinity conditions, while the E. americanum population preferred alkaline conditions. The findings of this study have improved the knowledge of the diversity, geographic distribution and evolution of bean-nodulating rhizobia in Mexico.
KW - Biogeography
KW - Mobility
KW - Phaseolus
KW - Phylogeny
KW - Rhizobia
KW - Soil pH
UR - http://www.scopus.com/inward/record.url?scp=84977814251&partnerID=8YFLogxK
U2 - 10.1016/j.syapm.2014.08.005
DO - 10.1016/j.syapm.2014.08.005
M3 - Artículo
C2 - 25294010
SN - 0723-2020
VL - 37
SP - 605
EP - 612
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 8
ER -