TY - JOUR
T1 - Magnetic extraction and purification of DNA from whole human blood using a γ-Fe2O3@Chitosan@Polyaniline hybrid nanocomposite
AU - Maciel, Bruna Gomes
AU - da Silva, Romário Justino
AU - Chávez-Guajardo, Alicia Elizabeth
AU - Medina-Llamas, Juan Carlos
AU - Alcaraz-Espinoza, José Jarib
AU - de Melo, Celso Pinto
N1 - Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/10/1
Y1 - 2018/10/1
N2 - We examined the properties of the nanocomposite γ-Fe2O3@Chi@Pani as an adsorbent of deoxyribonucleic acid (DNA). As a model system, we used an aqueous solution of salmon sperm DNA, whose decreasing concentration was followed by monitoring the 260 nm UV–vis absorption. After adjusting the data collected to a Langmuir isotherm curve, we estimated the adsorption capacity (qe) of the nanocomposite as 49.5 mg/g. We also observed that the kinetic model of the DNA capture presents a mixed character, with both chemical mechanisms and intraparticle diffusion processes involved. When the MNC was used to extract the DNA from complex samples (human blood), a capture rate of 80 ng/μL was achieved, with the collected fraction exhibiting good quality, as evaluated by PCR analysis and electrophoresis assays. These results suggest that the γ-Fe2O3@Chi@Pani nanocomposite is a promising adsorbent for use in protocols for purification of DNA from complex samples.
AB - We examined the properties of the nanocomposite γ-Fe2O3@Chi@Pani as an adsorbent of deoxyribonucleic acid (DNA). As a model system, we used an aqueous solution of salmon sperm DNA, whose decreasing concentration was followed by monitoring the 260 nm UV–vis absorption. After adjusting the data collected to a Langmuir isotherm curve, we estimated the adsorption capacity (qe) of the nanocomposite as 49.5 mg/g. We also observed that the kinetic model of the DNA capture presents a mixed character, with both chemical mechanisms and intraparticle diffusion processes involved. When the MNC was used to extract the DNA from complex samples (human blood), a capture rate of 80 ng/μL was achieved, with the collected fraction exhibiting good quality, as evaluated by PCR analysis and electrophoresis assays. These results suggest that the γ-Fe2O3@Chi@Pani nanocomposite is a promising adsorbent for use in protocols for purification of DNA from complex samples.
KW - Chitosan
KW - DNA extraction
KW - Human blood
KW - Maghemite
KW - Magnetic hybrid nanocomposite
KW - Polyaniline
UR - http://www.scopus.com/inward/record.url?scp=85047777084&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2018.05.034
DO - 10.1016/j.carbpol.2018.05.034
M3 - Artículo
C2 - 30007594
SN - 0144-8617
VL - 197
SP - 100
EP - 108
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -