TY - JOUR
T1 - Interleukin-13 receptor in psoriatic keratinocytes
T2 - Overexpression of the mRNA and underexpression of the protein
AU - Cancino-Díaz, Juan C.
AU - Reyes-Maldonado, Elba
AU - Bañuelos-Pánuco, Carmen A.
AU - Jiménez-Zamudio, Luis
AU - García-Latorre, Ethel
AU - León-Dorantes, Gladys
AU - Blancas-González, Fernando
AU - Paredes-Cabrera, Giovanna
AU - Cancino-Díaz, Mario Eugenio
N1 - Funding Information:
This work was supported in part by grants from Conacyt 38697-M, CGPI-IPN 200395 and CGPI-IPN 32.07. Juan C. Cancino-Díaz, received a scholarship from IPN, PIFI-IPN, and CONACYT. Elba Reyes-Maldonado, Luis Jiménez-Zamudio, Ethel García-Latorre are fellows COFAA and EDD, IPN. Mario E. Cancino-Díaz is fellow of COFAA and EDI, IPN.
PY - 2002
Y1 - 2002
N2 - Although several cytokines and their receptors have been involved in the development of psoriasis, the etiology is still unknown. In this study we looked for genes possibly involved in the disease by the reverse transcription-polymerase chain reaction differential display technique in lesional and nonlesional skin biopsies from psoriatic patients. We found the mRNA of the α1 chain of the interleukin-13 receptor expressed differentially in psoriatic biopsies. By reverse transcription-polymerase chain reaction, we confirmed an overexpression of the α1 chain of the IL-13 receptor and α chain of the interleukin-4 receptor mRNA in lesional skin psoriatic biopsies, when compared with skin biopsies from healthy subjects (p < 0.01). The nonlesional skin obtained from a region close to a lesional zone in psoriatic patients presented also an overexpression of these mRNA in 50% of the samples. Interleukin-13 and interleukin-4 were not detected either as mRNA or as the proteins in any of the biopsies from psoriatic patients or healthy subjects. A monoclonal antibody to the α1 chain of the interleukin-13 receptor detected the receptor in the epidermal keratinocytes of psoriatic patients and of healthy subjects; however, the positive antibody reaction was stronger in skin tissue from healthy subjects than in psoriatic lesional skin tissue (p < 0.01), although the mRNA was overexpressed. As interleukin-13 is a pleiotropic immunoregulatory cytokine with a variety of effects on different cell types, including monocytes, B lymphocytes, mast cells, and keratinocytes, we suggest, based on our results, that the interleukin-13 receptor possibly plays an important part in the early inflammatory process of psoriasis; however, its function is lost in the psoriatic keratinocytes.
AB - Although several cytokines and their receptors have been involved in the development of psoriasis, the etiology is still unknown. In this study we looked for genes possibly involved in the disease by the reverse transcription-polymerase chain reaction differential display technique in lesional and nonlesional skin biopsies from psoriatic patients. We found the mRNA of the α1 chain of the interleukin-13 receptor expressed differentially in psoriatic biopsies. By reverse transcription-polymerase chain reaction, we confirmed an overexpression of the α1 chain of the IL-13 receptor and α chain of the interleukin-4 receptor mRNA in lesional skin psoriatic biopsies, when compared with skin biopsies from healthy subjects (p < 0.01). The nonlesional skin obtained from a region close to a lesional zone in psoriatic patients presented also an overexpression of these mRNA in 50% of the samples. Interleukin-13 and interleukin-4 were not detected either as mRNA or as the proteins in any of the biopsies from psoriatic patients or healthy subjects. A monoclonal antibody to the α1 chain of the interleukin-13 receptor detected the receptor in the epidermal keratinocytes of psoriatic patients and of healthy subjects; however, the positive antibody reaction was stronger in skin tissue from healthy subjects than in psoriatic lesional skin tissue (p < 0.01), although the mRNA was overexpressed. As interleukin-13 is a pleiotropic immunoregulatory cytokine with a variety of effects on different cell types, including monocytes, B lymphocytes, mast cells, and keratinocytes, we suggest, based on our results, that the interleukin-13 receptor possibly plays an important part in the early inflammatory process of psoriasis; however, its function is lost in the psoriatic keratinocytes.
KW - IL-13
KW - Interleukin-13
KW - Kerationocytes
KW - Receptor psoriasis
UR - http://www.scopus.com/inward/record.url?scp=0036447301&partnerID=8YFLogxK
U2 - 10.1046/j.1523-1747.2002.19509.x
DO - 10.1046/j.1523-1747.2002.19509.x
M3 - Artículo
C2 - 12445201
SN - 0022-202X
VL - 119
SP - 1114
EP - 1120
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 5
ER -