TY - JOUR
T1 - Insightover0 lactobacillus plantarum 299v physicohemical characteristics of aggregation kinetics under starvation and different pH conditions estudio sobre las caracterÍsticas
AU - Melgar-Lalanne, G.
AU - Ley-Martínez, J.
AU - Azuara-Nieto, E.
AU - Téllez-Medina, D. I.
AU - Meza, T.
AU - González-González, C. R.
AU - Gutiérrez-López, G. F.
N1 - Publisher Copyright:
© 2019, Babes-Bolyai University, Alexandru Borza Botanic Garden. All Rights Reserved.
PY - 2019
Y1 - 2019
N2 - Bacterialaggregationisruledbyspecificandnonspecificadhesionmechanisms.FortheprobioticstrainLactobacillusplantarum 299v (Lp299v) the biochemistry of adhesion has been previously studied and S-layer proteins have been identified. However, physicochemical mechanisms which ruled the aggregation and adhesion phenomena able to change through the time under different pH conditions have not been completely elucidated. In this study we have described the non-specific adhesion mechanisms (hydrophobicity, zeta potential and morphology) of Lp299v auto-aggregates over time (24 h) at different pH levels (from 5.0 to 9.0) under starvation conditions. Auto-aggregation was time-dependent increasing up to 60% after 22 h at pH 5. On the other hand, the acidophilic nature of the Lp299v cell surface was confirmed showing higher affinity with chloroform than with n-hexadecane, while zeta potential was negative in all cases. Size and shape parameters were found to be time and pH dependent.AstrongPearsoncorrelation(0.991)wasobservedbetweencircularityandauto-aggregation.Inconclusion,Lp299v’s nonspecific adhesion mechanism was found to be more related to time than to pH levels, indicating that van der Waals and hydrophobic forces have the potential to dominate the auto-aggregation of Lp299v. Finally, the results showed that lower pH may promote auto-aggregation possibly easing the colonization at the upper level of the digestive system.
AB - Bacterialaggregationisruledbyspecificandnonspecificadhesionmechanisms.FortheprobioticstrainLactobacillusplantarum 299v (Lp299v) the biochemistry of adhesion has been previously studied and S-layer proteins have been identified. However, physicochemical mechanisms which ruled the aggregation and adhesion phenomena able to change through the time under different pH conditions have not been completely elucidated. In this study we have described the non-specific adhesion mechanisms (hydrophobicity, zeta potential and morphology) of Lp299v auto-aggregates over time (24 h) at different pH levels (from 5.0 to 9.0) under starvation conditions. Auto-aggregation was time-dependent increasing up to 60% after 22 h at pH 5. On the other hand, the acidophilic nature of the Lp299v cell surface was confirmed showing higher affinity with chloroform than with n-hexadecane, while zeta potential was negative in all cases. Size and shape parameters were found to be time and pH dependent.AstrongPearsoncorrelation(0.991)wasobservedbetweencircularityandauto-aggregation.Inconclusion,Lp299v’s nonspecific adhesion mechanism was found to be more related to time than to pH levels, indicating that van der Waals and hydrophobic forces have the potential to dominate the auto-aggregation of Lp299v. Finally, the results showed that lower pH may promote auto-aggregation possibly easing the colonization at the upper level of the digestive system.
KW - Aggregation
KW - Feret diameter
KW - Image analysis
KW - Probiotics
KW - Zeta potential
UR - http://www.scopus.com/inward/record.url?scp=85067868440&partnerID=8YFLogxK
U2 - 10.24275/UAM/IZT/DCBI/REVMEXINGQUIM/2019V18N1/MELGAR
DO - 10.24275/UAM/IZT/DCBI/REVMEXINGQUIM/2019V18N1/MELGAR
M3 - Artículo
AN - SCOPUS:85067868440
SN - 1665-2738
VL - 18
SP - 151
EP - 164
JO - Revista Mexicana de Ingeniera Quimica
JF - Revista Mexicana de Ingeniera Quimica
IS - 1
ER -