TY - JOUR
T1 - Innate immunity prevents tissue invasion by Entamoeba histolytica
AU - Shibayama, Mineko
AU - Rivera-Aguilar, Víctor
AU - Barbosa-Cabrera, Elizabeth
AU - Rojas-Hernández, Saúl
AU - Jarillo-Luna, Adriana
AU - Tsutsumi, Víctor
AU - Pacheco-Yepez, Judith
AU - Campos-Rodríguez, Rafael
PY - 2008/12
Y1 - 2008/12
N2 - Although innate and adaptive immunity both play a role in amoebiasis, the mechanisms involved in the elimination of Entamoeba histolytica are poorly understood. To provide more information about the innate immune mechanisms that may confer protection against invasive amoebiasis, we administered inflammatory substances (bacillus Calmette-Guérin, lipopolysaccharide, complete Freund's adjuvant, or mineral oil) into the peritoneum of hamsters. The animals were then challenged with pathogenic trophozoites of E. histolytica and, after 7 days, the protective host response was analysed. We found that the nonspecific inflammatory response induced in the peritoneum was sufficient to prevent liver invasion by E. histolytica. In vitro experiments showed that the killing of trophozoites was mediated by peritoneal macrophages and a protein of 68 kDa with peroxidase activity.
AB - Although innate and adaptive immunity both play a role in amoebiasis, the mechanisms involved in the elimination of Entamoeba histolytica are poorly understood. To provide more information about the innate immune mechanisms that may confer protection against invasive amoebiasis, we administered inflammatory substances (bacillus Calmette-Guérin, lipopolysaccharide, complete Freund's adjuvant, or mineral oil) into the peritoneum of hamsters. The animals were then challenged with pathogenic trophozoites of E. histolytica and, after 7 days, the protective host response was analysed. We found that the nonspecific inflammatory response induced in the peritoneum was sufficient to prevent liver invasion by E. histolytica. In vitro experiments showed that the killing of trophozoites was mediated by peritoneal macrophages and a protein of 68 kDa with peroxidase activity.
KW - Entamoeba histolytica
KW - Innate immunity
KW - Macrophages
KW - Nitric oxide
KW - Peroxidase
UR - http://www.scopus.com/inward/record.url?scp=58149172077&partnerID=8YFLogxK
U2 - 10.1139/W08-106
DO - 10.1139/W08-106
M3 - Artículo
C2 - 19096458
SN - 0008-4166
VL - 54
SP - 1032
EP - 1042
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
IS - 12
ER -