TY - JOUR
T1 - Influence of temperature on occlusion body production in a Mexican isolate of Spodoptera exigua multiple nucleopolyhedrovirus
AU - Luna-Espino, J. C.
AU - Martínez-Castillo, A. M.
AU - Ortiz, S. Ramos
AU - Pineda, S.
AU - Castrejón-Gómez, V. R.
N1 - Publisher Copyright:
© 2021 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2022
Y1 - 2022
N2 - In vivo production of baculoviruses requires optimisation studies for each host-virus system. Considering the potential of a Mexican isolate of Spodoptera exigua multiple nucleopolyhedrovirus (SeSIN6) for developing a biopesticide, here, a compartmentalised tray system and a cool-textured diet were used to determine the effect of incubation temperature on the pathogenicity, speed of kill, and production of occlusion bodies (OBs). All measurements were compared with a characterised American nucleopolyhedrovirus isolate (SeUS2). Incubation of fourth instar S. exigua at 20, 25, and 30°C did not significantly affect the mortality caused by either isolate, although a delay in virus infection was observed at 20°C. The OB production of both isolates in infected larvae was significantly lower at 20°C than at 25 and 30°C. At all temperatures, the OB production of the SeSIN6 isolate was higher than the SeUS2 isolate. The production of OBs per mg larva was significantly higher at 30°C for both isolates. A positive correlation was observed between larval weight gain and the production of OBs/larva at all temperatures, whereas a negative correlation was observed between larval gain weight and OBs/mg of larval weight in most of the treatments. We conclude that incubation temperatures of 25 and 30°C are optimal for OB production per larva in both isolates tested. Further studies are needed to examine other factors that can improve the production of Mexican SeMNPV isolates.
AB - In vivo production of baculoviruses requires optimisation studies for each host-virus system. Considering the potential of a Mexican isolate of Spodoptera exigua multiple nucleopolyhedrovirus (SeSIN6) for developing a biopesticide, here, a compartmentalised tray system and a cool-textured diet were used to determine the effect of incubation temperature on the pathogenicity, speed of kill, and production of occlusion bodies (OBs). All measurements were compared with a characterised American nucleopolyhedrovirus isolate (SeUS2). Incubation of fourth instar S. exigua at 20, 25, and 30°C did not significantly affect the mortality caused by either isolate, although a delay in virus infection was observed at 20°C. The OB production of both isolates in infected larvae was significantly lower at 20°C than at 25 and 30°C. At all temperatures, the OB production of the SeSIN6 isolate was higher than the SeUS2 isolate. The production of OBs per mg larva was significantly higher at 30°C for both isolates. A positive correlation was observed between larval weight gain and the production of OBs/larva at all temperatures, whereas a negative correlation was observed between larval gain weight and OBs/mg of larval weight in most of the treatments. We conclude that incubation temperatures of 25 and 30°C are optimal for OB production per larva in both isolates tested. Further studies are needed to examine other factors that can improve the production of Mexican SeMNPV isolates.
KW - Occlusion bodies
KW - bioinsecticides
KW - biological control
KW - pathogens
KW - pest control
UR - http://www.scopus.com/inward/record.url?scp=85117393570&partnerID=8YFLogxK
U2 - 10.1080/09583157.2021.1990855
DO - 10.1080/09583157.2021.1990855
M3 - Artículo
AN - SCOPUS:85117393570
SN - 0958-3157
VL - 32
SP - 223
EP - 235
JO - Biocontrol Science and Technology
JF - Biocontrol Science and Technology
IS - 2
ER -