TY - JOUR
T1 - Inflammatory response in human alveolar epithelial cells after TiO2 NPs or ZnO NPs exposure
T2 - Inhibition of surfactant protein A expression as an indicator for loss of lung function
AU - Jiménez-Chávez, A.
AU - Solorio-Rodríguez, A.
AU - Escamilla-Rivera, V.
AU - Leseman, D.
AU - Morales-Rubio, R.
AU - Uribe-Ramírez, M.
AU - Campos-Villegas, L.
AU - Medina-Ramírez, I. E.
AU - Arreola-Mendoza, L.
AU - Cassee, F. R.
AU - De Vizcaya-Ruiz, A.
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/8
Y1 - 2021/8
N2 - The increasing use of metal oxide nanoparticles (MONPs) as TiO2 NPs or ZnO NPs has led to environmental release and human exposure. The respiratory system, effects on lamellar bodies and surfactant protein A (SP-A) of pneumocytes, can be importantly affected. Exposure of human alveolar epithelial cells (A549) induced differential responses; a higher persistence of TiO2 in cell surface and uptake (measured by Atomic Force Microscopy) and sustained inflammatory response (by means of TNF-α, IL-10, and IL-6 release) and ROS generation were observed, whereas ZnO showed a modest response and low numbers in cell surface. A reduction in SP-A levels at 24 h of exposure to TiO2 NPs (concentration-dependent) or ZnO NPs (the higher concentration) was also observed, reversed by blocking the inflammatory response (by the inhibition of IL-6). Loss of SP-A represents a relevant target of MONPs-induced inflammatory response that could contribute to cellular damage and loss of lung function.
AB - The increasing use of metal oxide nanoparticles (MONPs) as TiO2 NPs or ZnO NPs has led to environmental release and human exposure. The respiratory system, effects on lamellar bodies and surfactant protein A (SP-A) of pneumocytes, can be importantly affected. Exposure of human alveolar epithelial cells (A549) induced differential responses; a higher persistence of TiO2 in cell surface and uptake (measured by Atomic Force Microscopy) and sustained inflammatory response (by means of TNF-α, IL-10, and IL-6 release) and ROS generation were observed, whereas ZnO showed a modest response and low numbers in cell surface. A reduction in SP-A levels at 24 h of exposure to TiO2 NPs (concentration-dependent) or ZnO NPs (the higher concentration) was also observed, reversed by blocking the inflammatory response (by the inhibition of IL-6). Loss of SP-A represents a relevant target of MONPs-induced inflammatory response that could contribute to cellular damage and loss of lung function.
KW - Interleukin 6
KW - MONPs biopersistence
KW - Surfactant protein A
KW - TiO NPs
KW - ZnO NPs
UR - http://www.scopus.com/inward/record.url?scp=85103792050&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2021.103654
DO - 10.1016/j.etap.2021.103654
M3 - Artículo
C2 - 33823299
AN - SCOPUS:85103792050
SN - 1382-6689
VL - 86
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
M1 - 103654
ER -