TY - JOUR
T1 - Infection of California sea lions (Zalophus californianus) with terrestrial Brucella spp
AU - Ávalos-Téllez, Rosalía
AU - Ramírez-Pfeiffer, Carlos
AU - Hernández-Castro, Rigoberto
AU - Díaz-Aparicio, Efrén
AU - Sánchez-Domínguez, Carlos
AU - Zavala-Norzagaray, Alan
AU - Arellano-Reynoso, Beatriz
AU - Suárez-Güemes, Francisco
AU - Aguirre, A. Alonso
AU - Aurioles-Gamboa, David
N1 - Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Infections with Brucella ceti and pinnipedialis are prevalent in marine mammals worldwide. A total of 22 California sea lions (Zalophus californianus) were examined to determine their exposure to Brucella spp. at San Esteban Island in the Gulf of California, Mexico, in June and July 2011. Although samples of blood, vaginal mucus and milk cultured negative for these bacteria, the application of rose Bengal, agar gel immunodiffusion, PCR and modified fluorescence polarization assays found that five animals (22.7%) had evidence of exposure to Brucella strains. The data also suggested that in two of these five sea lions the strains involved were of terrestrial origin, a novel finding in marine mammals. Further work will be required to validate and determine the epidemiological significance of this finding.
AB - Infections with Brucella ceti and pinnipedialis are prevalent in marine mammals worldwide. A total of 22 California sea lions (Zalophus californianus) were examined to determine their exposure to Brucella spp. at San Esteban Island in the Gulf of California, Mexico, in June and July 2011. Although samples of blood, vaginal mucus and milk cultured negative for these bacteria, the application of rose Bengal, agar gel immunodiffusion, PCR and modified fluorescence polarization assays found that five animals (22.7%) had evidence of exposure to Brucella strains. The data also suggested that in two of these five sea lions the strains involved were of terrestrial origin, a novel finding in marine mammals. Further work will be required to validate and determine the epidemiological significance of this finding.
KW - Agar gel immunodiffusion
KW - Brucella spp.
KW - California sea lion
KW - Fluorescence polarization
KW - PCR
KW - Rose bengal test
UR - http://www.scopus.com/inward/record.url?scp=84912561722&partnerID=8YFLogxK
U2 - 10.1016/j.tvjl.2014.06.021
DO - 10.1016/j.tvjl.2014.06.021
M3 - Artículo
C2 - 25066000
SN - 1090-0233
VL - 202
SP - 198
EP - 200
JO - Veterinary Journal
JF - Veterinary Journal
IS - 1
ER -