TY - JOUR
T1 - In vivo gastroprotective and antidepressant effects of iridoids, verbascoside and tenuifloroside from castilleja tenuiflora benth
AU - López-Rodríguez, Ricardo
AU - Herrera-Ruiz, Maribel
AU - Trejo-Tapia, Gabriela
AU - Domínguez-Mendoza, Blanca Eda
AU - González-Cortazar, Manasés
AU - Zamilpa, Alejandro
N1 - Publisher Copyright:
© 2019 by the authors.
PY - 2019/4/2
Y1 - 2019/4/2
N2 - Stress is an important factor in the etiology of some illnesses such as gastric ulcers and depression. Castilleja tenuiflora Benth. (Orobanchaceae) is used in Mexican traditional medicine for the treatment of gastrointestinal diseases and nervous disorders. Previous studies indicated that organic extracts from C. tenuiflora had gastroprotective effects and antidepressant activity. In this study, we aimed to evaluate the gastroprotective and antidepressant activity of fractions and isolated compounds from the methanolic extract (MECt) of C. tenuiflora in stressed mice. Chromatographic fractionation of MECt produced four fractions (FCt-1, FCt-2, CFt-3, and FCt-4) as well as four bioactive compounds which were identified using TLC, HPLC and NMR analyses. The cold restraint stress (CRS)-induced gastric ulcer model followed by the tail suspension test and the forced swim test were used to evaluate the gastroprotective effect and antidepressant activity of the extract fractions. FCt-2 and FCt-3 at 100 mg/kg had significant gastroprotective and antidepressant effects. All isolated compounds (verbascoside, teniufloroside and mixture geniposide/ musseanoside) displayed gastroprotective effects and antidepressant activity at 1 or 2 mg/kg. The above results allow us to conclude that these polyphenols and iridoids from C. tenuiflora are responsible for the gastroprotective and antidepressant effects.
AB - Stress is an important factor in the etiology of some illnesses such as gastric ulcers and depression. Castilleja tenuiflora Benth. (Orobanchaceae) is used in Mexican traditional medicine for the treatment of gastrointestinal diseases and nervous disorders. Previous studies indicated that organic extracts from C. tenuiflora had gastroprotective effects and antidepressant activity. In this study, we aimed to evaluate the gastroprotective and antidepressant activity of fractions and isolated compounds from the methanolic extract (MECt) of C. tenuiflora in stressed mice. Chromatographic fractionation of MECt produced four fractions (FCt-1, FCt-2, CFt-3, and FCt-4) as well as four bioactive compounds which were identified using TLC, HPLC and NMR analyses. The cold restraint stress (CRS)-induced gastric ulcer model followed by the tail suspension test and the forced swim test were used to evaluate the gastroprotective effect and antidepressant activity of the extract fractions. FCt-2 and FCt-3 at 100 mg/kg had significant gastroprotective and antidepressant effects. All isolated compounds (verbascoside, teniufloroside and mixture geniposide/ musseanoside) displayed gastroprotective effects and antidepressant activity at 1 or 2 mg/kg. The above results allow us to conclude that these polyphenols and iridoids from C. tenuiflora are responsible for the gastroprotective and antidepressant effects.
KW - Antidepressant
KW - Castilleja tenuiflora benth
KW - Cold-restraint stress
KW - Gastroprotective
KW - Tenuifloroside
KW - Verbascoside
UR - http://www.scopus.com/inward/record.url?scp=85063719690&partnerID=8YFLogxK
U2 - 10.3390/molecules24071292
DO - 10.3390/molecules24071292
M3 - Artículo
C2 - 30987044
AN - SCOPUS:85063719690
SN - 1420-3049
VL - 24
JO - Molecules
JF - Molecules
IS - 7
M1 - 1292
ER -