TY - JOUR
T1 - In vivo anti-inflammatory and anti-ulcerogenic activities of extracts from wild growing and in vitro plants of Castilleja tenuiflora Benth. (Orobanchaceae)
AU - Sanchez, Paul Mauricio
AU - Villarreal, María Luisa
AU - Herrera-Ruiz, Maribel
AU - Zamilpa, Alejandro
AU - Jiménez-Ferrer, Enrique
AU - Trejo-Tapia, Gabriela
N1 - Funding Information:
This research was financed by the Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional (SIP-IPN, Grants 20110153 , 20120745 and 20131786 ) and Fondo de Investigación Básica del Consejo Nacional de Ciencia y Tecnología-México (CONACYT, Grant 100202 ). PMSO is indebted to CONACyT and Programa Institucional de Formación de Investigadores (PIFI-IPN) for the awarded doctoral fellowship.
PY - 2013/12/12
Y1 - 2013/12/12
N2 - Ethnopharmacological relevance Castilleja tenuiflora Benth. (Orobanchaceae) is a perennial shrub used since the 16th century in Mexican traditional medicine for the treatment of a number of health disorders including inflammation, stomach pain and tumors. The aim of the present study was to evaluate the cytotoxic, anti-inflammatory and anti-ulcerogenic activities of ethyl acetate (EaE), methanol (ME) and aqueous extracts (AE) of Castilleja tenuiflora wild grown (CtW) and in vitro plants (CtIv). Material and method Phytochemical analysis of the phenylethanoid glycoside (PhG) and iridoid glycoside (IG) components was carried out by chromatographic methods. In vitro cytotoxic activity of the extracts was evaluated in the following four carcinoma cell lines: colon (HF-6), breast (MCF-7), prostate (PC-3), and nasopharyngeal (KB). The topical anti-inflammatory activity was evaluated in mouse ear edema induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). Anti-ulcerogenic activity was evaluated in rats using an absolute ethanol-induced acute gastric ulcer model. Results The main compounds in the extracts were isoverbascoside, verbascoside and aucubin and their concentration depended both on the solvent used and on the plant material origin. None of the extracts showed cytotoxicity against the tested cell lines. In contrast, CtWEaE, CtWAE and CtIvEaE (1.6 mg/ear) showed moderate anti-inflammatory activity similar to dexamethasone (1 mg/ear) with a 38.2, 39.3 and 49.1% decrease of inflammation, respectively. CtWEaE and CtIvEaE (100 mg/kg) showed high anti-ulcerogenic activity with 88.3 and 83.1% inhibition, respectively, compared to famotidine (20 mg/kg, 32.8% inhibition). Conclusion Castilleja tenuiflora extracts provided significant gastric protection in an acute ulcer induction model and topical anti-inflammatory activity in a mouse ear edema model. These activities are related to verbascoside and may explain the traditional use of Castilleja tenuiflora in the treatment of anti-inflammatory and gastrointestinal disorders. Cultured Castilleja tenuiflora plants (in vitro) exhibited pharmacological activities and also have the potential to produce bioactive compounds.
AB - Ethnopharmacological relevance Castilleja tenuiflora Benth. (Orobanchaceae) is a perennial shrub used since the 16th century in Mexican traditional medicine for the treatment of a number of health disorders including inflammation, stomach pain and tumors. The aim of the present study was to evaluate the cytotoxic, anti-inflammatory and anti-ulcerogenic activities of ethyl acetate (EaE), methanol (ME) and aqueous extracts (AE) of Castilleja tenuiflora wild grown (CtW) and in vitro plants (CtIv). Material and method Phytochemical analysis of the phenylethanoid glycoside (PhG) and iridoid glycoside (IG) components was carried out by chromatographic methods. In vitro cytotoxic activity of the extracts was evaluated in the following four carcinoma cell lines: colon (HF-6), breast (MCF-7), prostate (PC-3), and nasopharyngeal (KB). The topical anti-inflammatory activity was evaluated in mouse ear edema induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). Anti-ulcerogenic activity was evaluated in rats using an absolute ethanol-induced acute gastric ulcer model. Results The main compounds in the extracts were isoverbascoside, verbascoside and aucubin and their concentration depended both on the solvent used and on the plant material origin. None of the extracts showed cytotoxicity against the tested cell lines. In contrast, CtWEaE, CtWAE and CtIvEaE (1.6 mg/ear) showed moderate anti-inflammatory activity similar to dexamethasone (1 mg/ear) with a 38.2, 39.3 and 49.1% decrease of inflammation, respectively. CtWEaE and CtIvEaE (100 mg/kg) showed high anti-ulcerogenic activity with 88.3 and 83.1% inhibition, respectively, compared to famotidine (20 mg/kg, 32.8% inhibition). Conclusion Castilleja tenuiflora extracts provided significant gastric protection in an acute ulcer induction model and topical anti-inflammatory activity in a mouse ear edema model. These activities are related to verbascoside and may explain the traditional use of Castilleja tenuiflora in the treatment of anti-inflammatory and gastrointestinal disorders. Cultured Castilleja tenuiflora plants (in vitro) exhibited pharmacological activities and also have the potential to produce bioactive compounds.
KW - Castilleja tenuiflora
KW - Cytotoxicity
KW - Gastric ulcer
KW - Iridoid glycosides
KW - Phenylethanoid glycosides
KW - TPA-inflammation
UR - http://www.scopus.com/inward/record.url?scp=84889086618&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2013.10.002
DO - 10.1016/j.jep.2013.10.002
M3 - Artículo
C2 - 24145005
SN - 0378-8741
VL - 150
SP - 1032
EP - 1037
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 3
ER -