In Vitro Screening of Bioactive Compounds in some Gluten-Free Plants

Jerzy Drzewiecki; Alma Leticia Martinez-Ayala; María A. Lozano-Grande; Hanna Leontowicz; Maria Leontowicz; Zenon Jastrzebski; Pawel Pasko; Shela Gorinstein

doi:10.1007/s12010-018-2772-9

In Vitro Screening of Bioactive Compounds in some Gluten-Free Plants

Jerzy Drzewiecki, Alma Leticia Martinez-Ayala, María A. Lozano-Grande, Hanna Leontowicz, Maria Leontowicz, Zenon Jastrzebski, Pawel Pasko, Shela Gorinstein

Centro de Desarrollo de Productos Bióticos (CEPROBI)

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

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Resumen

Electrophoretic, antioxidant, and FTIR profiles of some varieties of amaranth, quinoa, and buckwheat seeds and their by products were compared. Water extracts of these products were evaluated by the Folin–Ciocalteau method in order to determine total phenolic content. The antioxidant activities were determined by 2,2′-azobis-2-methyl-propanimidamide, ferric-reducing/antioxidant power, and cupric reducing antioxidant capacity radical scavenging assays. FTIR spectra showed the secondary structure of pseudocereals in the ranges of amides I, II, and III shifts. Results of evaluated methods could be used to control several products (seeds, flours, extracts, flakes, roasting) with high phenolic content and antioxidant activity suitable for supplementation in food applications. [Figure not available: see fulltext.]

Idioma original	Inglés
Páginas (desde-hasta)	847-860
Número de páginas	14
Publicación	Applied Biochemistry and Biotechnology
Volumen	186
N.º	4
DOI	https://doi.org/10.1007/s12010-018-2772-9
Estado	Publicada - 1 dic. 2018

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title = "In Vitro Screening of Bioactive Compounds in some Gluten-Free Plants",

abstract = "Electrophoretic, antioxidant, and FTIR profiles of some varieties of amaranth, quinoa, and buckwheat seeds and their by products were compared. Water extracts of these products were evaluated by the Folin–Ciocalteau method in order to determine total phenolic content. The antioxidant activities were determined by 2,2′-azobis-2-methyl-propanimidamide, ferric-reducing/antioxidant power, and cupric reducing antioxidant capacity radical scavenging assays. FTIR spectra showed the secondary structure of pseudocereals in the ranges of amides I, II, and III shifts. Results of evaluated methods could be used to control several products (seeds, flours, extracts, flakes, roasting) with high phenolic content and antioxidant activity suitable for supplementation in food applications. [Figure not available: see fulltext.]",

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AU - Drzewiecki, Jerzy

AU - Martinez-Ayala, Alma Leticia

AU - Lozano-Grande, María A.

AU - Leontowicz, Hanna

AU - Leontowicz, Maria

AU - Jastrzebski, Zenon

AU - Pasko, Pawel

AU - Gorinstein, Shela

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Electrophoretic, antioxidant, and FTIR profiles of some varieties of amaranth, quinoa, and buckwheat seeds and their by products were compared. Water extracts of these products were evaluated by the Folin–Ciocalteau method in order to determine total phenolic content. The antioxidant activities were determined by 2,2′-azobis-2-methyl-propanimidamide, ferric-reducing/antioxidant power, and cupric reducing antioxidant capacity radical scavenging assays. FTIR spectra showed the secondary structure of pseudocereals in the ranges of amides I, II, and III shifts. Results of evaluated methods could be used to control several products (seeds, flours, extracts, flakes, roasting) with high phenolic content and antioxidant activity suitable for supplementation in food applications. [Figure not available: see fulltext.]

AB - Electrophoretic, antioxidant, and FTIR profiles of some varieties of amaranth, quinoa, and buckwheat seeds and their by products were compared. Water extracts of these products were evaluated by the Folin–Ciocalteau method in order to determine total phenolic content. The antioxidant activities were determined by 2,2′-azobis-2-methyl-propanimidamide, ferric-reducing/antioxidant power, and cupric reducing antioxidant capacity radical scavenging assays. FTIR spectra showed the secondary structure of pseudocereals in the ranges of amides I, II, and III shifts. Results of evaluated methods could be used to control several products (seeds, flours, extracts, flakes, roasting) with high phenolic content and antioxidant activity suitable for supplementation in food applications. [Figure not available: see fulltext.]

KW - Bioactivity

KW - Electrophoretic separation

KW - FTIR patterns

KW - Proteins

KW - Pseudocereals

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SN - 0273-2289

VL - 186

SP - 847

EP - 860

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

IS - 4

ER -

In Vitro Screening of Bioactive Compounds in some Gluten-Free Plants

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