Impaired T helper cell responses in human immunodeficiency virus-exposed uninfected newborns

Yesenia Brito-Pérez, Rodrigo T. Camacho-Pacheco, Noemi Plazola-Camacho, Diana Soriano-Becerril, Irma A. Coronado-Zarco, Gabriela Arreola-Ramírez, Gabriela González-Pérez, Alma Herrera-Salazar, Julio Flores-González, Mextli Y. Bermejo-Haro, Brenda G. Casorla-Cervantes, Ismael A. Soto-López, Jessica Hernández-Pineda, Claudia Sandoval-Montes, Sandra Rodríguez-Martínez, Ricardo Figueroa-Damian, Ismael Mancilla-Herrera

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

4 Citas (Scopus)

Resumen

Introduction: HIV-exposed uninfected (HEU) newborns suffer from higher risks of opportunistic infections during the first months of life compared to HIV-unexposed uninfected (HUU) newborns. Alterations in thymic mass, amounts of T helper (Th) cells, T-cell receptor diversity, and activation markers have been found in HEU newborns, suggesting alterations in T cell ontogeny and differentiation. However, little is known about the ability of these cells to produce specialized Th responses from CD4+ T cells. Method: To characterize the Th cell profile, we evaluated the frequency of Th1 (CD183+CD194CD196/CXCR3+CCR4CCR6), Th2 (CD183CD194+CD196/CXCR3CCR4+CCR6), Th17 (CD183CD194+CD196+/CXCR3CCR4+CCR6+), and CD4+CD25++ blood T-cell phenotypes in 50 HEU and 25 HUU newborns. Early activation markers on CD4+ T cells and the Th cytokine profile produced from mononuclear cells under polyclonal T cell stimulation were also studied. Additionally, we probed the ability of CD4+ T cells to differentiate into interferon (IFN)-γ-producing Th1 CD4+ T cells in vitro. Results: Lower percentages of differentiated Th1, Th2, Th17, and CD4+CD25++ T cells were found in blood from HEU newborns than in blood from HUU newborns. However, polyclonally stimulated Th cells showed a similar ability to express CD69 and CD279 but produced less secreted interleukin (IL)-2 and IL-4. Interestingly, under Th1 differentiation conditions, the percentages of CD4+IFN-γ+ T cells and soluble IFN-γ were higher in HEU newborns than in HUU newborns. Conclusion: HEU neonates are born with reduced proportions of differentiated Th1/Th2/Th17 and CD4+CD25++ T cells, but the intrinsic abilities of CD4+ T cells to acquire a Th1 profile are not affected by the adverse maternal milieu during development.

Idioma originalInglés
Páginas (desde-hasta)1541-1553
Número de páginas13
PublicaciónImmunity Inflammation and Disease
Volumen9
N.º4
DOI
EstadoPublicada - dic. 2021

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