Identification of two E-boxes that negatively modulate the activity of MyoD on the alpha-sarcoglycan core promoter

Paul Delgado-Olguín, J. Manuel Hernández-Hernández, Fabio Salamanca, Félix Recillas-Targa, Ramón M. Coral-Vázquez

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

6 Citas (Scopus)

Resumen

The α-SG promoter is composed of a plethora of cis-regulatory elements, whose individual contribution to α-SG gene expression modulation remains unknown. We have identified a negative regulatory element in the α-SG distal promoter including two conserved E-boxes (E1 and E2), which interact with MyoD. We found that E1 and E2 negatively modulate the transactivation potential of MyoD on the α-SG core promoter. Moreover, such negative effect is mainly mediated by E2, which is surrounded by conserved nucleotides conferring MyoD binding capacity. Our results suggest that modulation of MyoD activity by E1, and particularly E2, contributes to the negative regulation of α-SG gene expression during myogenic differentiation.

Idioma originalInglés
Páginas (desde-hasta)74-80
Número de páginas7
PublicaciónBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volumen1779
N.º1
DOI
EstadoPublicada - ene. 2008
Publicado de forma externa

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