TY - JOUR
T1 - Identification of two E-boxes that negatively modulate the activity of MyoD on the alpha-sarcoglycan core promoter
AU - Delgado-Olguín, Paul
AU - Hernández-Hernández, J. Manuel
AU - Salamanca, Fabio
AU - Recillas-Targa, Félix
AU - Coral-Vázquez, Ramón M.
N1 - Funding Information:
We thank to Georgina Guerrero Avendaño for technical assistance. We thank to Benoit G, Bruneau for his help with experiments and critical reading of the manuscript, Jeffrey F. Dilworth (Ottawa Health Research Institute) and Robin Meech (The Scripps Research Institute) for providing pFB-FL-MyoD/E47 and pCDNA-MyoD vectors, respectively. P.D.O was supported by the Consejo Nacional de Ciencia y Tecnología (CONACyT) (Grant 158524), the Dirección General de Estudios de Posgrado, UNAM, and the IMSS fellowship award. R.C.V. is supported by the Association Française contre les Myopathies (Grant MNM 2000 Groupe 1). F.R.T. is supported by CONACyT (Grants 33863-N and 42653-Q), PAPIIT-DGAPA (IN203200 and IN209403), The Third World Academy of Sciences (TWAS, Grant 01-055RG/BIO/LA) and Fundación Miguel Alemán.
PY - 2008/1
Y1 - 2008/1
N2 - The α-SG promoter is composed of a plethora of cis-regulatory elements, whose individual contribution to α-SG gene expression modulation remains unknown. We have identified a negative regulatory element in the α-SG distal promoter including two conserved E-boxes (E1 and E2), which interact with MyoD. We found that E1 and E2 negatively modulate the transactivation potential of MyoD on the α-SG core promoter. Moreover, such negative effect is mainly mediated by E2, which is surrounded by conserved nucleotides conferring MyoD binding capacity. Our results suggest that modulation of MyoD activity by E1, and particularly E2, contributes to the negative regulation of α-SG gene expression during myogenic differentiation.
AB - The α-SG promoter is composed of a plethora of cis-regulatory elements, whose individual contribution to α-SG gene expression modulation remains unknown. We have identified a negative regulatory element in the α-SG distal promoter including two conserved E-boxes (E1 and E2), which interact with MyoD. We found that E1 and E2 negatively modulate the transactivation potential of MyoD on the α-SG core promoter. Moreover, such negative effect is mainly mediated by E2, which is surrounded by conserved nucleotides conferring MyoD binding capacity. Our results suggest that modulation of MyoD activity by E1, and particularly E2, contributes to the negative regulation of α-SG gene expression during myogenic differentiation.
KW - Core promoter
KW - MyoD transactivation
KW - α-sarcoglycan promoter
UR - http://www.scopus.com/inward/record.url?scp=39449112791&partnerID=8YFLogxK
U2 - 10.1016/j.bbagrm.2007.09.002
DO - 10.1016/j.bbagrm.2007.09.002
M3 - Artículo
C2 - 18078839
SN - 1874-9399
VL - 1779
SP - 74
EP - 80
JO - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms
JF - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms
IS - 1
ER -