TY - JOUR
T1 - Identification of phenolic compounds from pollen extracts using capillary electrophoresis-electrospray time-of-flight mass spectrometry
AU - Arráez-Román, D.
AU - Zurek, G.
AU - Bäßmann, C.
AU - Almaraz-Abarca, N.
AU - Quirantes, R.
AU - Segura-Carretero, A.
AU - Fernández-Gutiérrez, A.
N1 - Funding Information:
Acknowledgements The author DAR gratefully acknowledges the financial support of a postdoctoral contract from the University of Granada (Perfeccionamiento de Doctores) by Bruker Daltonik GmbH and the financial support from the Ministerio de Educación y Ciencia, Spain (project CTQ2005-01914/BQU).
PY - 2007/11
Y1 - 2007/11
N2 - In this work, a new, easy and rapid method of analyzing phenolic compounds in pollen extract, based on capillary electrophoresis coupled with electrospray ionization time-of-flight-mass spectrometry (CE-ESI-TOF-MS), has been developed. A systematic investigation of separation parameters has been performed with respect to resolution, sensitivity, analysis time and peak shape. The electrophoretic parameters and electrospray conditions must be optimized to obtain reproducible analyses. Using this method, several important phenolic compounds such as acetin-glucoside, 7-O-methylherbacetin-3-sophoroside, galloyl-glucose, quercetin-3-sophoroside, apigenin-6,8-di-C-glycoside, quercetin-3-rutinoside, genistein-7-O-β-D-glucoside, luteolin-7-O- glucoside, apigenin-7-O-glucoside and 2′,4′,6′-trihydroxy- 3′-formyldihydrochalcone have been determined directly from pollen extract. The efficiency, the rapidity, the small amounts of sample required, and the high resolution of CE coupled with the sensitivity, the selectivity, the accurate masses and the true isotopic patterns obtained using TOF-MS point to the potential of this approach for identifying the phenolic compounds present in pollen.
AB - In this work, a new, easy and rapid method of analyzing phenolic compounds in pollen extract, based on capillary electrophoresis coupled with electrospray ionization time-of-flight-mass spectrometry (CE-ESI-TOF-MS), has been developed. A systematic investigation of separation parameters has been performed with respect to resolution, sensitivity, analysis time and peak shape. The electrophoretic parameters and electrospray conditions must be optimized to obtain reproducible analyses. Using this method, several important phenolic compounds such as acetin-glucoside, 7-O-methylherbacetin-3-sophoroside, galloyl-glucose, quercetin-3-sophoroside, apigenin-6,8-di-C-glycoside, quercetin-3-rutinoside, genistein-7-O-β-D-glucoside, luteolin-7-O- glucoside, apigenin-7-O-glucoside and 2′,4′,6′-trihydroxy- 3′-formyldihydrochalcone have been determined directly from pollen extract. The efficiency, the rapidity, the small amounts of sample required, and the high resolution of CE coupled with the sensitivity, the selectivity, the accurate masses and the true isotopic patterns obtained using TOF-MS point to the potential of this approach for identifying the phenolic compounds present in pollen.
KW - Capillary electrophoresis
KW - Electrospray ionization time-of-flight mass spectrometry
KW - Phenolic compounds
KW - Pollen
UR - http://www.scopus.com/inward/record.url?scp=36849068833&partnerID=8YFLogxK
U2 - 10.1007/s00216-007-1611-6
DO - 10.1007/s00216-007-1611-6
M3 - Artículo
SN - 1618-2642
VL - 389
SP - 1909
EP - 1917
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 6
ER -