TY - JOUR
T1 - Functional properties and microstructure of leuconostoc citreum and lactobacillus casei shirota exposed to habanero pepper extract and inhibition of staphylococcus aureus
AU - Peredo-Lovillo, A.
AU - Dorantes-Alvarez, L.
AU - Hernández-Sánchez, H.
AU - Ribas-Aparicio, R. M.
AU - Cauich-Sánchez, P. I.
AU - Aparicio-Ozores, G.
N1 - Publisher Copyright:
© 2019, Universidad Autonoma Metropolitana Iztapalapa. All rights reserved.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - The effects of habanero pepper extract on Staphylococcus aureus, Lactobacillus casei Shirota and Leuconostoc citreum were studied. The extract contained 8.71 mg/g of capsaicin and 4.72 mg/g of dihydrocapsaicin as well as minor concentrations of gallic, chlorogenic, caffeic, p-coumaric acids and quercetin, determined by HPLC. Inhibition of S. aureus at 4 h was observed with a death rate of-1.53 h-1. While, L. casei Shirota showed a survival of 4.15x105 CFU/mL and L. citreum of 2.20x105 CFU/mL. When L. casei Shirota was exposed 12 h to the MRS-50% extract, an elongation of the cells was observed and the Feret’s diameter increased from 3.3 to 21.6 µm; showing blistering on the cell surface. Regarding to L. citreum, an increase of 70% in cell area was observed, maintaining its circularity; also showed an improve in mucin adhesion ability of 4.42x105 CFU/mL, increasing its survival to bile salts by 85% and the synthesis of L36 ribosomal protein, corroborating the presence of rpmJ gene that encodes for this protein.
AB - The effects of habanero pepper extract on Staphylococcus aureus, Lactobacillus casei Shirota and Leuconostoc citreum were studied. The extract contained 8.71 mg/g of capsaicin and 4.72 mg/g of dihydrocapsaicin as well as minor concentrations of gallic, chlorogenic, caffeic, p-coumaric acids and quercetin, determined by HPLC. Inhibition of S. aureus at 4 h was observed with a death rate of-1.53 h-1. While, L. casei Shirota showed a survival of 4.15x105 CFU/mL and L. citreum of 2.20x105 CFU/mL. When L. casei Shirota was exposed 12 h to the MRS-50% extract, an elongation of the cells was observed and the Feret’s diameter increased from 3.3 to 21.6 µm; showing blistering on the cell surface. Regarding to L. citreum, an increase of 70% in cell area was observed, maintaining its circularity; also showed an improve in mucin adhesion ability of 4.42x105 CFU/mL, increasing its survival to bile salts by 85% and the synthesis of L36 ribosomal protein, corroborating the presence of rpmJ gene that encodes for this protein.
KW - Capsaicinoids
KW - Functional properties
KW - Lactobacillus casei Shirota
KW - Leuconostoc citreum
KW - Morphostructure
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=85068466644&partnerID=8YFLogxK
U2 - 10.24275/UAM/IZT/DCBI/REVMEXINGQUIM/2019V18N1/PEREDO
DO - 10.24275/UAM/IZT/DCBI/REVMEXINGQUIM/2019V18N1/PEREDO
M3 - Artículo
AN - SCOPUS:85068466644
SN - 1665-2738
VL - 18
SP - 115
EP - 129
JO - Revista Mexicana de Ingeniera Quimica
JF - Revista Mexicana de Ingeniera Quimica
IS - 1
ER -