TY - JOUR
T1 - Functional characterization of the three genes encoding 1-deoxy-D-xylulose 5-phosphate synthase in maize
AU - Cordoba, Elizabeth
AU - Porta, Helena
AU - Arroyo, Analilia
AU - San Román, Carolina
AU - Medina, Luis
AU - Rodríguez-Concepción, Manuel
AU - León, Patricia
N1 - Funding Information:
We are grateful to Dr Virginia Walbot and Dr. Mari Salmi for their helpful comments and Dr Axel Tiessen for providing us with some maize tissues. We thank Andrés Saralegui for his help with the confocal microscopy. We gratefully acknowledge the maize genomics database for providing maize lines and the EST clones used in this work. This work was supported by grants from CONACYT (40501-Q), DGAPA IN204503-3, BASF, and the Howard Hughes Medical Institution to PL and BIO200-00432 and CSD2007-00036 to MRC. Sequence data from this article have been deposited in GenBank.
PY - 2011/3
Y1 - 2011/3
N2 - The 1-deoxy-D-xylulose 5-phosphate synthase (DXS) enzyme catalyses the first biosynthetic step of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. In plants the MEP pathway is involved in the synthesis of the common precursors to the plastidic isoprenoids, isopentenyl diphosphate and dimethylallyl diphosphate, in plastids. DXS is recognized as limiting this pathway and is a potential target for manipulation to increase various isoprenoids such as carotenoids. In Zea mays three dxs genes exist that encode plastid-targeted functional enzymes. Evidence is provided that these genes represent phylogenetically distinctive clades conserved among plants preceding monocot-dicot divergence. There is differential accumulation for each dxs gene transcript, during development and in response to external signals such as light. At the protein level, the analysis demonstrates that in Z. mays, DXS protein is feedback regulated in response to the inhibition of the pathway flow. The results support that the multilevel regulation of DXS activity is conserved in evolution.
AB - The 1-deoxy-D-xylulose 5-phosphate synthase (DXS) enzyme catalyses the first biosynthetic step of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. In plants the MEP pathway is involved in the synthesis of the common precursors to the plastidic isoprenoids, isopentenyl diphosphate and dimethylallyl diphosphate, in plastids. DXS is recognized as limiting this pathway and is a potential target for manipulation to increase various isoprenoids such as carotenoids. In Zea mays three dxs genes exist that encode plastid-targeted functional enzymes. Evidence is provided that these genes represent phylogenetically distinctive clades conserved among plants preceding monocot-dicot divergence. There is differential accumulation for each dxs gene transcript, during development and in response to external signals such as light. At the protein level, the analysis demonstrates that in Z. mays, DXS protein is feedback regulated in response to the inhibition of the pathway flow. The results support that the multilevel regulation of DXS activity is conserved in evolution.
KW - 1-Deoxy-D-xylulose 5-phosphate synthase (DXS)
KW - 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway
KW - isoprenoid biosynthesis
KW - maize
KW - post-transcriptional regulation
UR - http://www.scopus.com/inward/record.url?scp=79952836026&partnerID=8YFLogxK
U2 - 10.1093/jxb/erq393
DO - 10.1093/jxb/erq393
M3 - Artículo
SN - 0022-0957
VL - 62
SP - 2023
EP - 2038
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 6
ER -