TY - JOUR
T1 - Fluorescent Gold Nanoparticle-Based Bioconjugate for the Detection of Salmonella
AU - Ríos-Corripio, M. A.
AU - Arcila-Lozano, L. S.
AU - Garcia-Perez, B. E.
AU - Jaramillo-Flores, M. E.
AU - Hernández-Pérez, A. D.
AU - Carlos-Martínez, A.
AU - Rosales-Perez, M.
AU - Rojas-López, M.
N1 - Publisher Copyright:
© 2016, Copyright © Taylor & Francis Group, LLC.
PY - 2016/8/12
Y1 - 2016/8/12
N2 - An easily prepared fluorescent bioconjugate colloidal solution based on gold nanoparticles is reported for the detection of Salmonella. This bioconjugate was prepared by conjugation of staphylococcal protein A on the surface of gold nanoparticles, followed by biofunctionalization over this layer with fluorescein isothiocyanate-labeled antibodies. The surface plasmon resonance band observed in the ultraviolet–visible spectrum of the gold nanoparticles showed a shift to low energy after covering the surface with protein A and a broadening of the band after biofunctionalization with the antibody. Surface-enhanced infrared spectroscopy showed the absorption bands of the conjugate and bioconjugate due to their proximity to the nanoparticles. In particular, vibrations at 1649, 1588, and 1400 cm−1 were from C˭O, N–H, and C–NH2 groups from the staphylococcal protein A covering the gold nanoparticles. The final bioconjugate colloidal solution was also used as probe to detecting Salmonella by confocal microscopy of solutions containing 1E3 and 1E5 CFU/ml. The total time required to detect these bacteria was less than 1 h. Fluorescent emission of the bacillus suggests that this methodology may have potential to develop a universal method for detecting different bacteria.
AB - An easily prepared fluorescent bioconjugate colloidal solution based on gold nanoparticles is reported for the detection of Salmonella. This bioconjugate was prepared by conjugation of staphylococcal protein A on the surface of gold nanoparticles, followed by biofunctionalization over this layer with fluorescein isothiocyanate-labeled antibodies. The surface plasmon resonance band observed in the ultraviolet–visible spectrum of the gold nanoparticles showed a shift to low energy after covering the surface with protein A and a broadening of the band after biofunctionalization with the antibody. Surface-enhanced infrared spectroscopy showed the absorption bands of the conjugate and bioconjugate due to their proximity to the nanoparticles. In particular, vibrations at 1649, 1588, and 1400 cm−1 were from C˭O, N–H, and C–NH2 groups from the staphylococcal protein A covering the gold nanoparticles. The final bioconjugate colloidal solution was also used as probe to detecting Salmonella by confocal microscopy of solutions containing 1E3 and 1E5 CFU/ml. The total time required to detect these bacteria was less than 1 h. Fluorescent emission of the bacillus suggests that this methodology may have potential to develop a universal method for detecting different bacteria.
KW - Bioconjugate
KW - Salmonella
KW - biosensors
KW - gold nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84978120917&partnerID=8YFLogxK
U2 - 10.1080/00032719.2015.1128944
DO - 10.1080/00032719.2015.1128944
M3 - Artículo
SN - 0003-2719
VL - 49
SP - 1862
EP - 1873
JO - Analytical Letters
JF - Analytical Letters
IS - 12
ER -