TY - JOUR
T1 - Extraendothelial and constitutive COX-2 expression is involved in the contractile effect of angiotensin II in the rat aorta
AU - Castillo-Hernández, M. C.
AU - Martinez-Godinez, M. A.
AU - Guevara-Balcazar, G.
AU - Miliar-Garcia, A.
AU - Mancilla, J.
AU - Lopez-Mayorga, R. M.
AU - Castillo-Henkel, E. F.
AU - Castillo-Henkel, C.
PY - 2010/10
Y1 - 2010/10
N2 - 1 The role of the extraendothelial and constitutive isoforms of cyclo-oxygenase-2 (COX-2) in the contractile effect of angiotensin II (Ang II) was investigated using thoracic and abdominal aortic rings without endothelium from young Wistar rats. 2 Ang II elicited similar contractions in both aortic segments, and the effect was inhibited by pretreatment with NS398 (a selective COX-2 inhibitor) but not SC-560 [selective cyclo-oxygenase-1 (COX-1) inhibitor]. 3 COX-2 mRNA was expressed under basal conditions in both aortic segments. Additionally, Ang II increased COX-2 mRNA expression in the abdominal but not the thoracic segment, while cycloheximide (a protein synthesis inhibitor) did not affect the contractile response to Ang II in either of the two segments; this suggests that the effect is not associated with de novo COX-2 synthesis. 4 In conclusion, the basal amount of COX-2 found in aortic smooth muscle cells is sufficient to explain the production of the prostanoids related to the contractile effect of Ang II. The production of these prostanoids, which are derived from constitutive COX-2, occurs independently of the endothelium vascular system.
AB - 1 The role of the extraendothelial and constitutive isoforms of cyclo-oxygenase-2 (COX-2) in the contractile effect of angiotensin II (Ang II) was investigated using thoracic and abdominal aortic rings without endothelium from young Wistar rats. 2 Ang II elicited similar contractions in both aortic segments, and the effect was inhibited by pretreatment with NS398 (a selective COX-2 inhibitor) but not SC-560 [selective cyclo-oxygenase-1 (COX-1) inhibitor]. 3 COX-2 mRNA was expressed under basal conditions in both aortic segments. Additionally, Ang II increased COX-2 mRNA expression in the abdominal but not the thoracic segment, while cycloheximide (a protein synthesis inhibitor) did not affect the contractile response to Ang II in either of the two segments; this suggests that the effect is not associated with de novo COX-2 synthesis. 4 In conclusion, the basal amount of COX-2 found in aortic smooth muscle cells is sufficient to explain the production of the prostanoids related to the contractile effect of Ang II. The production of these prostanoids, which are derived from constitutive COX-2, occurs independently of the endothelium vascular system.
KW - Angiotensin II
KW - COX-2
KW - abdominal aorta
KW - smooth muscle
KW - thoracic aorta
UR - http://www.scopus.com/inward/record.url?scp=77956412445&partnerID=8YFLogxK
U2 - 10.1111/j.1474-8673.2010.00457.x
DO - 10.1111/j.1474-8673.2010.00457.x
M3 - Artículo
C2 - 20626388
SN - 1474-8665
VL - 30
SP - 205
EP - 211
JO - Autonomic and Autacoid Pharmacology
JF - Autonomic and Autacoid Pharmacology
IS - 4
ER -