TY - JOUR
T1 - Expression of alternative NADH dehydrogenases (NDH-2) in the phytopathogenic fungus Ustilago maydis
AU - Matuz-Mares, Deyamira
AU - Matus-Ortega, Genaro
AU - Cárdenas-Monroy, Christian
AU - Romero-Aguilar, Lucero
AU - Villalobos-Rocha, Juan Carlos
AU - Vázquez-Meza, Héctor
AU - Guerra-Sánchez, Guadalupe
AU - Peña-Díaz, Antonio
AU - Pardo, Juan Pablo
N1 - Publisher Copyright:
© 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
PY - 2018/8
Y1 - 2018/8
N2 - Type 2 alternative NADH dehydrogenases (NDH-2) participate indirectly in the generation of the electrochemical proton gradient by transferring electrons from NADH and NADPH into the ubiquinone pool. Due to their structural simplicity, alternative NADH dehydrogenases have been proposed as useful tools for gene therapy of cells with defects in the respiratory complex I. In this work, we report the presence of three open reading frames, which correspond to NDH-2 genes in the genome of Ustilago maydis. These three genes were constitutively transcribed in cells cultured in YPD and minimal medium with glucose, ethanol, or lactate as carbon sources. Proteomic analysis showed that only two of the three NDH-2 were associated with isolated mitochondria in all culture media. Oxygen consumption by permeabilized cells using NADH or NADPH was different for each condition, opening the possibility of posttranslational regulation. We confirmed the presence of both external and internal NADH dehydrogenases, as well as an external NADPH dehydrogenase insensitive to calcium. Higher oxygen consumption rates were observed during the exponential growth phase, suggesting that the activity of NADH and NADPH dehydrogenases is coupled to the dynamics of cell growth.
AB - Type 2 alternative NADH dehydrogenases (NDH-2) participate indirectly in the generation of the electrochemical proton gradient by transferring electrons from NADH and NADPH into the ubiquinone pool. Due to their structural simplicity, alternative NADH dehydrogenases have been proposed as useful tools for gene therapy of cells with defects in the respiratory complex I. In this work, we report the presence of three open reading frames, which correspond to NDH-2 genes in the genome of Ustilago maydis. These three genes were constitutively transcribed in cells cultured in YPD and minimal medium with glucose, ethanol, or lactate as carbon sources. Proteomic analysis showed that only two of the three NDH-2 were associated with isolated mitochondria in all culture media. Oxygen consumption by permeabilized cells using NADH or NADPH was different for each condition, opening the possibility of posttranslational regulation. We confirmed the presence of both external and internal NADH dehydrogenases, as well as an external NADPH dehydrogenase insensitive to calcium. Higher oxygen consumption rates were observed during the exponential growth phase, suggesting that the activity of NADH and NADPH dehydrogenases is coupled to the dynamics of cell growth.
KW - NADH/NADPH quinone oxidoreductase
KW - Ustilago maydis
KW - alternative NAD(P)H dehydrogenases
KW - corn smut fungi
KW - electron transport chain
KW - gene expression
UR - http://www.scopus.com/inward/record.url?scp=85050949532&partnerID=8YFLogxK
U2 - 10.1002/2211-5463.12475
DO - 10.1002/2211-5463.12475
M3 - Artículo
C2 - 30221129
AN - SCOPUS:85050949532
SN - 2211-5463
VL - 8
SP - 1267
EP - 1279
JO - FEBS Open Bio
JF - FEBS Open Bio
IS - 8
ER -