TY - JOUR
T1 - Expression Analysis of Ni- and V-Associated Resistance Genes in a Bacillus megaterium Strain Isolated from a Mining Site
AU - Fierros Romero, Grisel
AU - Rivas Castillo, Andrea
AU - Gómez Ramírez, Marlenne
AU - Pless, Reynaldo
AU - Rojas Avelizapa, Norma
N1 - Publisher Copyright:
© 2016, Springer Science+Business Media New York.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Bacillus megaterium strain MNSH1-9K-1 was isolated from a mining site in Guanajuato, Mexico. This B. megaterium strain presented the ability to remove Ni and V from a spent catalyst. Also, its associated metal resistance genes nccA, hant, VAN2, and smtAB were previously identified by a PCR approach. The present study reports for the first time, in B. megaterium, the changes in the expression of the genes nccA (Ni–Co–Cd resistance); hant (high-affinity nickel transporter); smtAB, a metal-binding protein gene; and VAN2 (V resistance) after exposure to 200 ppm of Ni and 200 ppm of V during the stationary phase of the microorganism in PHGII liquid media. The data presented here may contribute to the knowledge of the genes involved in the Ni and V resistances of B. megaterium, and the possible pathways implicated in the Ni–V removal processes, which may be potentiated for the biological treatment of high metal content residues.
AB - Bacillus megaterium strain MNSH1-9K-1 was isolated from a mining site in Guanajuato, Mexico. This B. megaterium strain presented the ability to remove Ni and V from a spent catalyst. Also, its associated metal resistance genes nccA, hant, VAN2, and smtAB were previously identified by a PCR approach. The present study reports for the first time, in B. megaterium, the changes in the expression of the genes nccA (Ni–Co–Cd resistance); hant (high-affinity nickel transporter); smtAB, a metal-binding protein gene; and VAN2 (V resistance) after exposure to 200 ppm of Ni and 200 ppm of V during the stationary phase of the microorganism in PHGII liquid media. The data presented here may contribute to the knowledge of the genes involved in the Ni and V resistances of B. megaterium, and the possible pathways implicated in the Ni–V removal processes, which may be potentiated for the biological treatment of high metal content residues.
UR - http://www.scopus.com/inward/record.url?scp=84964310673&partnerID=8YFLogxK
U2 - 10.1007/s00284-016-1044-6
DO - 10.1007/s00284-016-1044-6
M3 - Artículo
C2 - 27107759
SN - 0343-8651
VL - 73
SP - 165
EP - 171
JO - Current Microbiology
JF - Current Microbiology
IS - 2
ER -