Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts

Karla Stephanie Martinez-Elizalde, Manuel Jimenez-Estrada, Cesar Mateo Flores, Luis Barbo Hernandez, Rocio Rosas-Lopez, Angel Duran-Diaz, Oscar J. Nieto-Yañez, Elizabeth Barbosa, Marco Aurelio Rodriguez-Monroy, Margarita Canales-Martinez

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4 Citas (Scopus)

Resumen

Background: The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species. Methods: Methanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected. Results: The Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC=4 mg/mL) and Vibrio cholera (MIC=4 mg/mL). The MeOH2 inhibited the DPPH radical (SC50=69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD50=22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid. Conclusions: The fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit.

Idioma originalInglés
Número de artículo74
PublicaciónBMC Complementary and Alternative Medicine
Volumen15
N.º1
DOI
EstadoPublicada - 21 mar. 2015

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