TY - JOUR
T1 - Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts
AU - Martinez-Elizalde, Karla Stephanie
AU - Jimenez-Estrada, Manuel
AU - Flores, Cesar Mateo
AU - Hernandez, Luis Barbo
AU - Rosas-Lopez, Rocio
AU - Duran-Diaz, Angel
AU - Nieto-Yañez, Oscar J.
AU - Barbosa, Elizabeth
AU - Rodriguez-Monroy, Marco Aurelio
AU - Canales-Martinez, Margarita
N1 - Publisher Copyright:
© Martinez-Elizalde et al.; licensee BioMed Central.
PY - 2015/3/21
Y1 - 2015/3/21
N2 - Background: The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species. Methods: Methanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected. Results: The Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC=4 mg/mL) and Vibrio cholera (MIC=4 mg/mL). The MeOH2 inhibited the DPPH radical (SC50=69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD50=22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid. Conclusions: The fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit.
AB - Background: The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species. Methods: Methanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected. Results: The Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC=4 mg/mL) and Vibrio cholera (MIC=4 mg/mL). The MeOH2 inhibited the DPPH radical (SC50=69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD50=22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid. Conclusions: The fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit.
KW - Anacardiaceae
KW - Antibacterial activity
KW - Cyrtocarpa pocera
KW - Medicinal fruit
UR - http://www.scopus.com/inward/record.url?scp=84925270592&partnerID=8YFLogxK
U2 - 10.1186/s12906-015-0602-y
DO - 10.1186/s12906-015-0602-y
M3 - Artículo
SN - 1472-6882
VL - 15
JO - BMC Complementary and Alternative Medicine
JF - BMC Complementary and Alternative Medicine
IS - 1
M1 - 74
ER -