TY - JOUR
T1 - EVALUATION OF THE ANTI-INFLAMMATORY CAPACITY OF BETA-SITOSTEROL IN RODENT ASSAYS
AU - Paniagua-Pérez, Rogelio
AU - Flores-Mondragón, Gabriela
AU - Reyes-Legorreta, Celia
AU - Herrera-López, Brígida
AU - Cervantes-Hernández, Isabel
AU - Madrigal-Santillán, Osiris
AU - Morales-González, José Antonio
AU - Álvarez-González, Isela
AU - Madrigal-Bujaidar, Eduardo
PY - 2017
Y1 - 2017
N2 - BACKGROUND: Beta-sitosterol (BS) is a compound discovered to be present in numerous plants. A number of interesting biomedical properties have been attributed to BS, including immuno-modulating and anti-inflammatory activities. Therefore, the aim of this report was to evaluate its anti-inflammatory capacity by applying various rodent experimental tests.METHODS: To carry out the objective of the study we applied the methods indicated here. Two of the adopted methods were based on the passive reverse Arthus reaction: the rat paw edema test and the rat pleurisy assay. We also applied two methods related with the non-specific acute inflammation: the mouse ear edema test, and the mouse mieloperoxidase activity assay.RESULTS: The results obtained in all tests established a significant anti-inflammatory potential of BS. In the rat paw edema test we found an inhibitory effect which goes from 50-70%; in the rat pleurisy assay our findings with respect to the volume of pleural exuded showed a reduction of 46%, as well as a 20% low amount of neutrophils in comparison with the level of the control group. In the mouse ear edema test we found a mean inflammatory inhibition of 75%, and with respect to mieloproxidase activity the results showed a significant inhibition induced by the three doses of BS.CONCLUSIONS: In the present study we determined a potent anti-inflammatory capacity of BS in specific and non-specific types of acute inflammation in rodents.
AB - BACKGROUND: Beta-sitosterol (BS) is a compound discovered to be present in numerous plants. A number of interesting biomedical properties have been attributed to BS, including immuno-modulating and anti-inflammatory activities. Therefore, the aim of this report was to evaluate its anti-inflammatory capacity by applying various rodent experimental tests.METHODS: To carry out the objective of the study we applied the methods indicated here. Two of the adopted methods were based on the passive reverse Arthus reaction: the rat paw edema test and the rat pleurisy assay. We also applied two methods related with the non-specific acute inflammation: the mouse ear edema test, and the mouse mieloperoxidase activity assay.RESULTS: The results obtained in all tests established a significant anti-inflammatory potential of BS. In the rat paw edema test we found an inhibitory effect which goes from 50-70%; in the rat pleurisy assay our findings with respect to the volume of pleural exuded showed a reduction of 46%, as well as a 20% low amount of neutrophils in comparison with the level of the control group. In the mouse ear edema test we found a mean inflammatory inhibition of 75%, and with respect to mieloproxidase activity the results showed a significant inhibition induced by the three doses of BS.CONCLUSIONS: In the present study we determined a potent anti-inflammatory capacity of BS in specific and non-specific types of acute inflammation in rodents.
KW - Beta-sitosterol
KW - anti-inflammatory assays
KW - at
KW - mouse
UR - http://www.scopus.com/inward/record.url?scp=85021858951&partnerID=8YFLogxK
U2 - 10.21010/ajtcam.v14i1.13
DO - 10.21010/ajtcam.v14i1.13
M3 - Artículo
C2 - 28480389
SN - 0189-6016
VL - 14
SP - 123
EP - 130
JO - African journal of traditional, complementary, and alternative medicines : AJTCAM
JF - African journal of traditional, complementary, and alternative medicines : AJTCAM
IS - 1
ER -