TY - JOUR
T1 - Evaluation of process involved in the production of aromatic compounds in Gram-negative bacteria isolated from vanilla (Vanilla planifolia ex. Andrews) beans
AU - Escobar-Muciño, E.
AU - Luna-Guevara, M. L.
AU - Ramos-Cassellis, M. E.
AU - Amador-Espejo, G. G.
AU - Castañeda-Lucio, M.
AU - Arenas-Hernández, M. M.P.
N1 - Publisher Copyright:
© 2019 The Society for Applied Microbiology
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Aim: The present investigation was aimed at isolating and identifying bacterial strains from cured vanilla beans. Additionally, the study focused on evaluating bacterial processes pertaining to the aromatic compounds production (ACP). Methods and Results: Three bacteria were isolated from Vanilla planifolia beans, previously subjected to the curing process. According to morphological, biochemical and 16S rRNA analysis, the strains were identified as Citrobacter sp., Enterobacter sp. and Pseudomonas sp. The polygalacturonase activity (PGA) was determined using the drop, cup-plate and DNS methods. Aromatic compounds production was analysed by cup-plate method using FA as substrate and quantified by high performance liquid chromatography (ppm), the functional groups of vanillic acid (VA) were identified by FT-IR and the aromatic compounds (AC) resistance was determined and reported as minimum inhibitory concentration. Citrobacter sp., Enterobacter sp. and Pseudomonas showed PGA (70·31 ± 364, 76·07 ± 12·47 and 51 ± 10·92 U ml−1 respectively), were producers of VA (3·23 ± 0·49, 324 ± 41 and 265·99 ± 11·61 ppm respectively) and were resistant to AC. Conclusions: The Gram-negative bacteria isolated from V. planifolia beans were responsible for ACP. Significance and Impact of the Study: This is the first evidence for the role of Gram-negative bacterial isolates from cured Mexican V. planifolia beans in the process related to ACP.
AB - Aim: The present investigation was aimed at isolating and identifying bacterial strains from cured vanilla beans. Additionally, the study focused on evaluating bacterial processes pertaining to the aromatic compounds production (ACP). Methods and Results: Three bacteria were isolated from Vanilla planifolia beans, previously subjected to the curing process. According to morphological, biochemical and 16S rRNA analysis, the strains were identified as Citrobacter sp., Enterobacter sp. and Pseudomonas sp. The polygalacturonase activity (PGA) was determined using the drop, cup-plate and DNS methods. Aromatic compounds production was analysed by cup-plate method using FA as substrate and quantified by high performance liquid chromatography (ppm), the functional groups of vanillic acid (VA) were identified by FT-IR and the aromatic compounds (AC) resistance was determined and reported as minimum inhibitory concentration. Citrobacter sp., Enterobacter sp. and Pseudomonas showed PGA (70·31 ± 364, 76·07 ± 12·47 and 51 ± 10·92 U ml−1 respectively), were producers of VA (3·23 ± 0·49, 324 ± 41 and 265·99 ± 11·61 ppm respectively) and were resistant to AC. Conclusions: The Gram-negative bacteria isolated from V. planifolia beans were responsible for ACP. Significance and Impact of the Study: This is the first evidence for the role of Gram-negative bacterial isolates from cured Mexican V. planifolia beans in the process related to ACP.
KW - Citrobacter sp.
KW - Enterobacter sp.
KW - Pseudomonas sp.
KW - aromatic compounds resistant
KW - bioconversion
KW - pectinolytic activity
KW - vanilla
KW - vanillic acid
UR - http://www.scopus.com/inward/record.url?scp=85076732340&partnerID=8YFLogxK
U2 - 10.1111/jam.14537
DO - 10.1111/jam.14537
M3 - Artículo
C2 - 31793107
SN - 1364-5072
VL - 128
SP - 1086
EP - 1098
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
IS - 4
ER -