TY - GEN
T1 - Evaluation of physical properties and antibacterial activity of bioactive compounds-loaded chitosan nanoparticles
AU - Correa-Pacheco, Zormy Nacary
AU - Bautista-Baños, Silvia
AU - Hernández-López, Mónica
AU - Corona-Rangel, María Luisa
N1 - Publisher Copyright:
© 2018 Trans Tech Publications, Switzerland.
PY - 2018
Y1 - 2018
N2 - Bioactive compounds such as essential oils (EO), botanical extracts and natural resins are well known to have beneficial properties. Among these properties are their antibacterial activity. A disadvantage of these compounds is that they are volatile. Therefore, encapsulation is a good way to overcome this problem. In this study, the morphology, particle size distribution, Zeta potential and microbiological activity of chitosan nanoparticles incorporated with three different bioactive compounds having antimicrobial properties: ethanol extract of propolis, thyme essential oil and ethanol extract of Byrsonima crassifolia (L.) Kunth were evaluated. Nanoparticles were synthesized using the nanoprecipitation method. The morphology was observed using transmission electron microscopy (TEM). Also, particle size distribution and Zeta potential were measured. Results show spherical in shape nanoparticles. Thyme essential oil-loaded chitosan nanoparticles (TEO-CSNPs) showed the smallest particle size and highest stability as assessed by Zeta potential measurement, followed in stability by ethanol extract of propolis-loaded chitosan nanoparticles (EEP-CSNPs), ethanol extract of Byrsonima crassifolia (L.) Kunth (EEBC-CSNPs) and finally by chitosan nanoparticles (CSNPs). The antibacterial activity of the bioactive compounds-loaded chitosan nanoparticles was evaluated against Staphylococcus aureus. The highest antibacterial activity was observed for TEO-CSNPs with an inhibition halo (IH) value of 10.54±0.78 mm, followed by EEP-CSNPs (8.10±1.19 mm). EEBC-CSNPs and CSNPs did not show zone of inhibition. Bioactive compounds-loaded chitosan nanoparticles represents a good alternative for bacterial control of food borne pathogens in applications for fruits and vegetables conservation.
AB - Bioactive compounds such as essential oils (EO), botanical extracts and natural resins are well known to have beneficial properties. Among these properties are their antibacterial activity. A disadvantage of these compounds is that they are volatile. Therefore, encapsulation is a good way to overcome this problem. In this study, the morphology, particle size distribution, Zeta potential and microbiological activity of chitosan nanoparticles incorporated with three different bioactive compounds having antimicrobial properties: ethanol extract of propolis, thyme essential oil and ethanol extract of Byrsonima crassifolia (L.) Kunth were evaluated. Nanoparticles were synthesized using the nanoprecipitation method. The morphology was observed using transmission electron microscopy (TEM). Also, particle size distribution and Zeta potential were measured. Results show spherical in shape nanoparticles. Thyme essential oil-loaded chitosan nanoparticles (TEO-CSNPs) showed the smallest particle size and highest stability as assessed by Zeta potential measurement, followed in stability by ethanol extract of propolis-loaded chitosan nanoparticles (EEP-CSNPs), ethanol extract of Byrsonima crassifolia (L.) Kunth (EEBC-CSNPs) and finally by chitosan nanoparticles (CSNPs). The antibacterial activity of the bioactive compounds-loaded chitosan nanoparticles was evaluated against Staphylococcus aureus. The highest antibacterial activity was observed for TEO-CSNPs with an inhibition halo (IH) value of 10.54±0.78 mm, followed by EEP-CSNPs (8.10±1.19 mm). EEBC-CSNPs and CSNPs did not show zone of inhibition. Bioactive compounds-loaded chitosan nanoparticles represents a good alternative for bacterial control of food borne pathogens in applications for fruits and vegetables conservation.
KW - Antibacterial
KW - Byrsonima crassifolia
KW - Chitosan nanoparticles
KW - Propolis
KW - Thyme essential oil
UR - http://www.scopus.com/inward/record.url?scp=85059959737&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/MSF.936.3
DO - 10.4028/www.scientific.net/MSF.936.3
M3 - Contribución a la conferencia
SN - 9783035713787
T3 - Materials Science Forum
SP - 3
EP - 7
BT - Materials Engineering and Nanotechnology
A2 - Umemura, Kazuo
PB - Trans Tech Publications Ltd
T2 - 3rd International Conference on Materials Engineering and Nanotechnology, ICMEN 2018
Y2 - 19 July 2018 through 21 July 2018
ER -