TY - JOUR
T1 - (−)-Epicatechin protects from amebic liver abscess development in hamster
AU - Velásquez-Torres, Maritza
AU - Shibayama-Salas, Mineko
AU - Pacheco-Yépez, Judith
AU - Silva-Olivares, Angélica
AU - Sánchez-Monroy, Virginia
AU - Marchat, Laurence A.
AU - Rivera, Gildardo
AU - Ramírez-Moreno, Esther
N1 - Funding Information:
This work was supported by the Secretar?a de Investigaci?n y Posgrado, Instituto Polit?cnico Nacional (SIP-IPN)-Mexico (projects 20170802, 20180520) and Consejo Nacional de Ciencia y Tecnolog?a (CONACYT)-Mexico (project 285467). M. Vel?squez-Torres received financial support through the scholarship 20170802 granted by BEIFI-IPN, and the scholarship 615810 granted by (CONACYT)-Mexico. Participants received any of the following supports: COFAA-IPN, EDI-IPN and SNI-CONACyT.
Funding Information:
This work was supported by the Secretaría de Investigación y Posgrado, Instituto Politécnico Nacional (SIP-IPN)-Mexico (projects 20170802 , 20180520 ) and Consejo Nacional de Ciencia y Tecnología (CONACYT)-Mexico (project 285467 ). M. Velásquez-Torres received financial support through the scholarship 20170802 granted by BEIFI-IPN , and the scholarship 615810 granted by (CONACYT)-Mexico . Participants received any of the following supports: COFAA-IPN , EDI-IPN and SNI-CONACyT .
Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/5
Y1 - 2021/5
N2 - In this work the effect of (−)-epicatechin on the development of amebic liver abscess in hamsters was evaluated. (−)-epicatechin is a flavonoid present in plants that possesses various biological properties, including its activity against some protozoal parasites; however its antiamebic activity in a living model had not been evaluated. Syrian golden hamsters were intrahepatically inoculated with 1x106 E. histolytica trophozoites, three days after inoculation they received nine intraperitoneal doses of (−)-epicatechin (10 mg/100 g) every 48 h. Animals without treatments and treated with metronidazole were included as controls. Macroscopic characteristics of the hepatic abscess, histopathological analysis of the tissue and the levels of inflammatory cytokines were determined. (−)-epicatechin produced a decrease in liver abscess progression being observed only 9.49% of damage compared to 84% shown by untreated animals. During treatment with (−)-epicatechin hepatic tissue showed signs of liver repair and absence of amoebae. Additionally, (−)-epicatechin produced a modulating effect on inflammatory cytokines TNF-α, IL-1β and IL-10. All these events observed in animals treated with (−)-epicatechin could contribute to the elimination of trophozoites and liver healing.
AB - In this work the effect of (−)-epicatechin on the development of amebic liver abscess in hamsters was evaluated. (−)-epicatechin is a flavonoid present in plants that possesses various biological properties, including its activity against some protozoal parasites; however its antiamebic activity in a living model had not been evaluated. Syrian golden hamsters were intrahepatically inoculated with 1x106 E. histolytica trophozoites, three days after inoculation they received nine intraperitoneal doses of (−)-epicatechin (10 mg/100 g) every 48 h. Animals without treatments and treated with metronidazole were included as controls. Macroscopic characteristics of the hepatic abscess, histopathological analysis of the tissue and the levels of inflammatory cytokines were determined. (−)-epicatechin produced a decrease in liver abscess progression being observed only 9.49% of damage compared to 84% shown by untreated animals. During treatment with (−)-epicatechin hepatic tissue showed signs of liver repair and absence of amoebae. Additionally, (−)-epicatechin produced a modulating effect on inflammatory cytokines TNF-α, IL-1β and IL-10. All these events observed in animals treated with (−)-epicatechin could contribute to the elimination of trophozoites and liver healing.
KW - (-)-Epicatechin
KW - Amebic liver abscess
KW - Antiamebic activity
KW - Flavonoids
UR - http://www.scopus.com/inward/record.url?scp=85103611464&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2021.108103
DO - 10.1016/j.exppara.2021.108103
M3 - Artículo
C2 - 33771537
AN - SCOPUS:85103611464
SN - 0014-4894
VL - 224
JO - Experimental Parasitology
JF - Experimental Parasitology
M1 - 108103
ER -
