(−)-Epicatechin modulates the expression of myomiRs implicated in exercise response in mouse skeletal muscle

Carlos Palma-Flores, Paola B. Zárate-Segura, J. Manuel Hernández-Hernández, Sergio de los Santos, Andrea S. Tejeda-Gómez, Luis Javier Cano-Martínez, Patricia Canto, Jorge Omar Garcia-Rebollar, Ramón M. Coral-Vázquez

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

The flavanol (−)-epicatechin has exercise-mimetic properties. Besides, several miRNAs play a role in modulating the adaptation of the muscle to different training protocols. However, notwithstanding all information, few studies aimed to determine if (−)-epicatechin can modify the expression of miRNAs related to skeletal muscle development and regeneration. Mice were treated for fifteen days by oral gavage with the flavanol (−)-epicatechin. After treatment, the quadriceps of the mice was dissected, and total RNA was extracted. The expression level of miR-133, -204, -206, -223, -486, and -491 was analyzed by qRT-PCR. We also used bioinformatic analysis to predict the participation of these miRNAs in different skeletal muscle signal transduction pathways. Additionally, we analyzed the level of the myogenic proteins MyoD and myogenin by Western blot and measured the cross-sectional area of muscle fibers stained with E&H. (−)-Epicatechin upregulated the expression of miR-133, -204, -206, -223, and -491 significantly, which was associated with an increase in the level of the myogenic proteins MyoD and Myogenin and an augment in the fiber size. The bioinformatics analysis showed that the studied miRNAs might participate in different signal transduction pathways related to muscle development and adaptation. Our results showed that (−)-epicatechin upregulated miRNAs that participate in skeletal exercise muscle adaptation, induced muscle hypertrophy, and increased the level of myogenic proteins MyoD and MyoG.

Idioma originalInglés
Número de artículo146907
PublicaciónGene
Volumen849
DOI
EstadoPublicada - 15 ene. 2023

Huella

Profundice en los temas de investigación de '(−)-Epicatechin modulates the expression of myomiRs implicated in exercise response in mouse skeletal muscle'. En conjunto forman una huella única.

Citar esto