TY - JOUR
T1 - Entamoeba histolytica
T2 - Immunohistochemical study of hepatic amoebiasis in mouse. Neutrophils and nitric oxide as possible factors of resistance
AU - Jarillo-Luna, R. A.
AU - Campos-Rodríguez, R.
AU - Tsutsumi, V.
N1 - Funding Information:
We are grateful to Mr. J. Ayala-Dávila, Ms. A. Ramı́rez-Rosales, Ms. C. Guadarrama-Olmos, Ms. M. Miranda-Sánchez for their valuable technical assistance and Mr. H. Garcı́a-Farfán for preparation of photographic material. This work was supported in part by a grant from CONACYT (No. M9608-3533) (México).
PY - 2002/5/1
Y1 - 2002/5/1
N2 - Studies in mice have not rendered conclusive data on cell and humoral factors to support the resistance of this rodent to Entamoeba histolytica infection. In Balb/c and C3H/HeJ mice inoculated with live or fixed trophozoites, we studied the evolution of the hepatic lesion, the kinetics of inflammatory cells, and the participation of some humoral factors in the development of the hepatic amoebic lesion. From the first hour, amoebae were surrounded by neutrophils containing inducible nitric oxide synthase (iNOS); macrophages also expressing iNOS appeared lately, whereas NK cells were not part of the inflammatory infiltrates. On the fourth day, neutrophils, macrophages, T and B lymphocytes, plasma cells, and some NK cells limited the lesions and anti-amoeba antibodies appeared when most parasites had been eliminated. Therefore, the resistance of the mice to E. histolytica probably lies in non-specific immune responses, among which the activation of neutrophils and the production of nitric oxide (NO) may be important amoebicide factors.
AB - Studies in mice have not rendered conclusive data on cell and humoral factors to support the resistance of this rodent to Entamoeba histolytica infection. In Balb/c and C3H/HeJ mice inoculated with live or fixed trophozoites, we studied the evolution of the hepatic lesion, the kinetics of inflammatory cells, and the participation of some humoral factors in the development of the hepatic amoebic lesion. From the first hour, amoebae were surrounded by neutrophils containing inducible nitric oxide synthase (iNOS); macrophages also expressing iNOS appeared lately, whereas NK cells were not part of the inflammatory infiltrates. On the fourth day, neutrophils, macrophages, T and B lymphocytes, plasma cells, and some NK cells limited the lesions and anti-amoeba antibodies appeared when most parasites had been eliminated. Therefore, the resistance of the mice to E. histolytica probably lies in non-specific immune responses, among which the activation of neutrophils and the production of nitric oxide (NO) may be important amoebicide factors.
UR - http://www.scopus.com/inward/record.url?scp=0036591273&partnerID=8YFLogxK
U2 - 10.1016/S0014-4894(02)00021-8
DO - 10.1016/S0014-4894(02)00021-8
M3 - Artículo
SN - 0014-4894
VL - 101
SP - 40
EP - 56
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 1
ER -