TY - JOUR
T1 - Encapsulation efficiency of CdSe/ZnS quantum dots by liposomes determined by thermal lens microscopy
AU - Batalla, Jessica
AU - Cabrera, Humberto
AU - Martín-Martínez, Eduardo San
AU - Korte, Dorota
AU - Calderón, Antonio
AU - Marín, Ernesto
N1 - Publisher Copyright:
© 2015 Optical Society of America.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - In this study the encapsulation of core shell carboxyl CdSe/ZnS quantum dots (QDs) by phospholipids liposome complexes is presented. It makes the quantum dots water soluble and photo-stable. Fluorescence selfquenching of the QDs inside the liposomes was observed. Therefore, the thermal lens microscopy (TLM) was found to be an useful tool for measuring the encapsulation efficiency of the QDs by the liposomes, for which an optimum value of 36% was determined. The obtained limit of detection (LOD) for determining QDs concentration by TLM was 0.13 nM. Moreover, the encapsulated QDs showed no prominent cytotoxicity toward Breast cancer cells line MDA-MB-231. This study was supported by UVvisible spectroscopy, high resolution transmission electron microscopy (HRTEM) and dynamic light scattering measurements (DLS).
AB - In this study the encapsulation of core shell carboxyl CdSe/ZnS quantum dots (QDs) by phospholipids liposome complexes is presented. It makes the quantum dots water soluble and photo-stable. Fluorescence selfquenching of the QDs inside the liposomes was observed. Therefore, the thermal lens microscopy (TLM) was found to be an useful tool for measuring the encapsulation efficiency of the QDs by the liposomes, for which an optimum value of 36% was determined. The obtained limit of detection (LOD) for determining QDs concentration by TLM was 0.13 nM. Moreover, the encapsulated QDs showed no prominent cytotoxicity toward Breast cancer cells line MDA-MB-231. This study was supported by UVvisible spectroscopy, high resolution transmission electron microscopy (HRTEM) and dynamic light scattering measurements (DLS).
UR - http://www.scopus.com/inward/record.url?scp=84948746699&partnerID=8YFLogxK
U2 - 10.1364/BOE.6.003898
DO - 10.1364/BOE.6.003898
M3 - Artículo
SN - 2156-7085
VL - 6
SP - 3898
EP - 3906
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 10
M1 - A016
ER -