TY - JOUR
T1 - Effect of screening and subculture on the production of betaxanthins in Beta vulgaris L. var. 'Dark Detroit' callus culture
AU - Trejo-Tapia, G.
AU - Balcazar-Aguilar, J. B.
AU - Martínez-Bonfil, B.
AU - Salcedo-Morales, G.
AU - Jaramillo-Flores, M.
AU - Arenas-Ocampo, M. L.
AU - Jiménez-Aparicio, A.
N1 - Funding Information:
The authors want to thank Instituto Politecnico Nacional (SIP 2006) and National Council of Science and Technology (39562 Mexico) for funding this project.
PY - 2008/1
Y1 - 2008/1
N2 - Plant cell culture is an innovative technology to produce a variety of substances including natural dyes. Betaxanthins are considered food-safe nutraceutics pigments because exhibit antiradical and antioxidant activity. An important obstacle for developing large-scale production systems based in plant cells has been the instability of metabolite accumulation. In this work, a protocol was established to obtain yellow callus of B. vulgaris var. 'Dark Detroit'. Homogeneous and heterogeneous pigmented callus were obtained with yellow, red, orange and colorless phenotype. Particular attention was done to isolate and establish a yellow line. After continuous screening of the more intense yellow callus it was possible to increase the betaxanthins production 1.8-fold after 48 subcultures. Spectrophotometric and chromatographic analysis of the pigments, confirmed the presence of betaxanthins. HPLC analysis indicated two mainly distinct betaxanthins, vulgaxanthin I and II. B. vulgaris yellow callus line did not loose pigment production ability as a result of long-term subculture.
AB - Plant cell culture is an innovative technology to produce a variety of substances including natural dyes. Betaxanthins are considered food-safe nutraceutics pigments because exhibit antiradical and antioxidant activity. An important obstacle for developing large-scale production systems based in plant cells has been the instability of metabolite accumulation. In this work, a protocol was established to obtain yellow callus of B. vulgaris var. 'Dark Detroit'. Homogeneous and heterogeneous pigmented callus were obtained with yellow, red, orange and colorless phenotype. Particular attention was done to isolate and establish a yellow line. After continuous screening of the more intense yellow callus it was possible to increase the betaxanthins production 1.8-fold after 48 subcultures. Spectrophotometric and chromatographic analysis of the pigments, confirmed the presence of betaxanthins. HPLC analysis indicated two mainly distinct betaxanthins, vulgaxanthin I and II. B. vulgaris yellow callus line did not loose pigment production ability as a result of long-term subculture.
KW - Beta vulgaris
KW - Betaxanthins production
KW - Callus culture
KW - Long-term subculture
KW - The stable B. vulgaris cell culture technology developed could be an alternative process to obtain betaxanthins as nutraceutic pigments.
UR - http://www.scopus.com/inward/record.url?scp=36448959482&partnerID=8YFLogxK
U2 - 10.1016/j.ifset.2007.04.009
DO - 10.1016/j.ifset.2007.04.009
M3 - Artículo
SN - 1466-8564
VL - 9
SP - 32
EP - 36
JO - Innovative Food Science and Emerging Technologies
JF - Innovative Food Science and Emerging Technologies
IS - 1
ER -