TY - JOUR
T1 - Effect of keratinous waste addition on improvement of crude oil hydrocarbon removal by a hydrocarbon-degrading and keratinolytic mixed culture
AU - Cervantes-González, E.
AU - Rojas-Avelizapa, L. I.
AU - Cruz-Camarillo, R.
AU - Rojas-Avelizapa, N. G.
N1 - Funding Information:
This work was supported by grants, 20050273 and 20060201 from the Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional, and other financial support from the Instituto Mexicano del Petróleo. Elsa Cervantes Gonzalez was supported by the scholarship number 162631 from CONACyT. Norma G. Rojas, Luz I. Rojas and R. Cruz C are COFAA and EDI fellowships.
PY - 2009/12
Y1 - 2009/12
N2 - The aim of this work was to evaluate the effect of keratinous waste addition on oil-hydrocarbon removal, through a mixed culture of oil-degrading bacteria, with the ability to secrete keratinases. The mixed culture was grown in the media with oil, or oil supplemented with chicken-feathers as the keratinous waste. Residual oil-hydrocarbons were determined as total petroleum hydrocarbons (TPHs) and oil fractions and then quantified by GC-FID and GC-MS. Results showed that in presence of the keratinous waste, the removal of oil-hydrocarbons was 57,400 mg l-1, meanwhile the treatment without waste presented an oil-hydrocarbons removal of 35,600 mg l-1. The aliphatic fraction was the most removed in both treatments. In addition, chromatographic profiles indicated that the aliphatic fraction showed different degradation pattern; in the presence of keratinous wastes, the C18 to C28 compounds were preferably removed over the C10 to C17. The addition of keratinous waste not only improved the oil-hydrocarbons removal but, it changed the removal pattern of the target hydrocarbons.
AB - The aim of this work was to evaluate the effect of keratinous waste addition on oil-hydrocarbon removal, through a mixed culture of oil-degrading bacteria, with the ability to secrete keratinases. The mixed culture was grown in the media with oil, or oil supplemented with chicken-feathers as the keratinous waste. Residual oil-hydrocarbons were determined as total petroleum hydrocarbons (TPHs) and oil fractions and then quantified by GC-FID and GC-MS. Results showed that in presence of the keratinous waste, the removal of oil-hydrocarbons was 57,400 mg l-1, meanwhile the treatment without waste presented an oil-hydrocarbons removal of 35,600 mg l-1. The aliphatic fraction was the most removed in both treatments. In addition, chromatographic profiles indicated that the aliphatic fraction showed different degradation pattern; in the presence of keratinous wastes, the C18 to C28 compounds were preferably removed over the C10 to C17. The addition of keratinous waste not only improved the oil-hydrocarbons removal but, it changed the removal pattern of the target hydrocarbons.
KW - Chicken-feathers wastes
KW - Keratinolytic bacteria
KW - Oil removal
KW - Oil sorbents
UR - http://www.scopus.com/inward/record.url?scp=72149100472&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2009.06.014
DO - 10.1016/j.ibiod.2009.06.014
M3 - Artículo
SN - 0964-8305
VL - 63
SP - 1018
EP - 1022
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
IS - 8
ER -