TY - JOUR
T1 - Dystrophin isoform Dp71 is present in lamellipodia and focal complexes in human astrocytoma cells U-373 MG
AU - García-Tovar, Carlos G.
AU - Luna, José
AU - Mena, Raúl
AU - Soto-Zárate, Carlos I.
AU - Cortés, Rafael
AU - Pérez, Armando
AU - León-Avila, Gloria
AU - Mornet, Dominique
AU - Rendón, Alvaro
AU - Hernández, José Manuel
N1 - Funding Information:
This study was supported in part by The European Economic Community (EI-CT93-0098) and by a CONACYT scholarship (85542) granted to the first author. We are grateful to Fortunato Mote, David Pérez, Armando Sánchez and Ivan Vargas for their valuable technical support, and Esther Cid for her secretarial support. We also thank Imelda Saldaña, Alberto Martínez, Arnulfo Apango, Pedro Hernández and José Luis Zarco for assistance in bibliographic services.
PY - 2002
Y1 - 2002
N2 - Dp71 is the most abundant product of the dmd gene in the brain. There are at least 2 isoforms derived from alternative splicing of exon 78 (Dp71d, which contains exon 78 and Dp71f, the spliced isoform) but the precise localization and function of each isoform is still unknown. In the present study, we demonstrate by RT-PCR that the Dp71f isoform is present in an astrocytoma cell line U-373 MG, and its subcellular localization was determined in the cytoplasm, particularly in perinuclear areas, with lower amounts towards the periphery but increasing in the leader borders of lamellipodia and focal complexes. Double labeling indirect immunofluorescence showed that Dp71f colocalized with actin-like β-dystroglycan and β-1 integrin. We also demonstrated by triple labeling that Dp71f was colocalized with actin and two members of integrin complexes, α-actinin and vinculin, in focal complexes. Ventral plasma membranes were enriched and in those containing focal complex proteins, we found colocalization of Dp71f, actin and vinculin. It is concluded that U-373 MG cells express Dp71f as part of lamellipodia and focal complex proteins, and possibly connected via distroglycan complexes to integrin complexes.
AB - Dp71 is the most abundant product of the dmd gene in the brain. There are at least 2 isoforms derived from alternative splicing of exon 78 (Dp71d, which contains exon 78 and Dp71f, the spliced isoform) but the precise localization and function of each isoform is still unknown. In the present study, we demonstrate by RT-PCR that the Dp71f isoform is present in an astrocytoma cell line U-373 MG, and its subcellular localization was determined in the cytoplasm, particularly in perinuclear areas, with lower amounts towards the periphery but increasing in the leader borders of lamellipodia and focal complexes. Double labeling indirect immunofluorescence showed that Dp71f colocalized with actin-like β-dystroglycan and β-1 integrin. We also demonstrated by triple labeling that Dp71f was colocalized with actin and two members of integrin complexes, α-actinin and vinculin, in focal complexes. Ventral plasma membranes were enriched and in those containing focal complex proteins, we found colocalization of Dp71f, actin and vinculin. It is concluded that U-373 MG cells express Dp71f as part of lamellipodia and focal complex proteins, and possibly connected via distroglycan complexes to integrin complexes.
KW - Cell adhesion
KW - Dp71f
KW - Dystrophin
KW - Glycoprotein complex
KW - Integrin complex
UR - http://www.scopus.com/inward/record.url?scp=0036025152&partnerID=8YFLogxK
U2 - 10.1078/0065-1281-00654
DO - 10.1078/0065-1281-00654
M3 - Artículo
SN - 0065-1281
VL - 104
SP - 245
EP - 254
JO - Acta Histochemica
JF - Acta Histochemica
IS - 3
ER -