TY - JOUR
T1 - Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis in Coffea canephora
AU - Ayil-Gutiérrez, Benjamín
AU - Galaz-Ávalos, Rosa María
AU - Peña-Cabrera, Eduardo
AU - Loyola-Vargas, Víctor Manuel
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/12/3
Y1 - 2013/12/3
N2 - Most of the somatic embryogenesis (SE) process requires the presence, either before or during the embryogenic process, of at least one exogenous auxin. This exogenous auxin induces the presence of endogenous auxins, which appears to be essential for SE induction. We found that during the preincubation period of SE in Coffea canephora, there is an important increase in both free and conjugated indole-3-acetic acid (IAA), as well as indole-3-butyric acid. This increase is accompanied by an increase in the expression of YUCCA (CcYUC), TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (CcTAA1), and GRETCHEN HAGEN 3 (GH3) genes. On the other hand, most of the IAA compounds decreased during the induction of SE. The results presented in this research suggest that a balance between free IAA and its amide conjugates is necessary to allow the expression of SE-related genes.
AB - Most of the somatic embryogenesis (SE) process requires the presence, either before or during the embryogenic process, of at least one exogenous auxin. This exogenous auxin induces the presence of endogenous auxins, which appears to be essential for SE induction. We found that during the preincubation period of SE in Coffea canephora, there is an important increase in both free and conjugated indole-3-acetic acid (IAA), as well as indole-3-butyric acid. This increase is accompanied by an increase in the expression of YUCCA (CcYUC), TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (CcTAA1), and GRETCHEN HAGEN 3 (GH3) genes. On the other hand, most of the IAA compounds decreased during the induction of SE. The results presented in this research suggest that a balance between free IAA and its amide conjugates is necessary to allow the expression of SE-related genes.
KW - Auxins
KW - GH3
KW - Growth regulators
KW - Indole-3-acetic acid
KW - Somatic embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=84890943563&partnerID=8YFLogxK
U2 - 10.4161/psb.26998
DO - 10.4161/psb.26998
M3 - Artículo
C2 - 24299659
AN - SCOPUS:84890943563
SN - 1559-2316
VL - 8
JO - Plant Signaling and Behavior
JF - Plant Signaling and Behavior
IS - 11
M1 - e26998
ER -