Double herbicide-resistant biotypes of wild oat (Avena fatua) display characteristic metabolic fingerprints before and after applying ACCase- and ALS-inhibitors

Jesús R. Torres-García, J. Antonio Tafoya-Razo, Sabina Velázquez-Márquez, Axel Tiessen

Resultado de la investigación: Contribución a una revistaArtículo

3 Citas (Scopus)

Resumen

© 2018, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków. Plant herbicides inhibit specific enzymes of biosynthetic metabolism, such as acetyl-coenzyme A carboxylase (ACCase) and acetolactate synthase (ALS). Herbicide resistance can be caused by point mutations at the binding domains, catalytic sites and other regions within multimeric enzymes. Direct-injection electrospray mass spectrometry was used for high-throughput metabolic fingerprinting for finding significant differences among biotypes in response to herbicide application. A Mexican biotype of wild oat (Avena fatua) that displays multiple resistances to ACCase- and ALS-inhibiting herbicides was characterized. The dose–response test showed that the double-resistant biotype had a resistance index of 3.58 for pinoxaden and 3.53 for mesosulfuron-methyl. Resistance was accompanied by characteristic mutations at the site of action: an I-1781-L substitution occurred in the ACCase enzyme and an S-653-N mutation was identified within the ALS enzyme. Other mutations were also detected in the genes of the Mexican biotypes. The ionomic fingerprint showed that the multiple-resistant biotype had a markedly different metabolic pattern under control conditions and that this difference was accentuated after herbicide treatment. This demonstrates that single changes of amino acid sequences can produce several holistic modifications in the metabolism of resistant plants compared to susceptible plants. We conclude that in addition to genetic resistance, additional mechanisms of metabolic adaptation and detoxification can occur in multiple-resistant weed plants.
Idioma originalInglés estadounidense
PublicaciónActa Physiologiae Plantarum
DOI
EstadoPublicada - 1 jun 2018
Publicado de forma externa

Huella dactilar

Acetolactate Synthase
Coenzymes
Acetyl-CoA Carboxylase
Avena fatua
acetolactate synthase
acetyl-CoA carboxylase
Herbicides
Dermatoglyphics
biotypes
oats
herbicides
Enzymes
Mutation
enzymes
mutation
Metabolism
Herbicide Resistance
Plant Physiological Phenomena
Detoxification
Point Mutation

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title = "Double herbicide-resistant biotypes of wild oat (Avena fatua) display characteristic metabolic fingerprints before and after applying ACCase- and ALS-inhibitors",
abstract = "{\circledC} 2018, Franciszek G{\'o}rski Institute of Plant Physiology, Polish Academy of Sciences, Krak{\'o}w. Plant herbicides inhibit specific enzymes of biosynthetic metabolism, such as acetyl-coenzyme A carboxylase (ACCase) and acetolactate synthase (ALS). Herbicide resistance can be caused by point mutations at the binding domains, catalytic sites and other regions within multimeric enzymes. Direct-injection electrospray mass spectrometry was used for high-throughput metabolic fingerprinting for finding significant differences among biotypes in response to herbicide application. A Mexican biotype of wild oat (Avena fatua) that displays multiple resistances to ACCase- and ALS-inhibiting herbicides was characterized. The dose–response test showed that the double-resistant biotype had a resistance index of 3.58 for pinoxaden and 3.53 for mesosulfuron-methyl. Resistance was accompanied by characteristic mutations at the site of action: an I-1781-L substitution occurred in the ACCase enzyme and an S-653-N mutation was identified within the ALS enzyme. Other mutations were also detected in the genes of the Mexican biotypes. The ionomic fingerprint showed that the multiple-resistant biotype had a markedly different metabolic pattern under control conditions and that this difference was accentuated after herbicide treatment. This demonstrates that single changes of amino acid sequences can produce several holistic modifications in the metabolism of resistant plants compared to susceptible plants. We conclude that in addition to genetic resistance, additional mechanisms of metabolic adaptation and detoxification can occur in multiple-resistant weed plants.",
author = "Torres-Garc{\'i}a, {Jes{\'u}s R.} and Tafoya-Razo, {J. Antonio} and Sabina Vel{\'a}zquez-M{\'a}rquez and Axel Tiessen",
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Double herbicide-resistant biotypes of wild oat (Avena fatua) display characteristic metabolic fingerprints before and after applying ACCase- and ALS-inhibitors. / Torres-García, Jesús R.; Tafoya-Razo, J. Antonio; Velázquez-Márquez, Sabina; Tiessen, Axel.

En: Acta Physiologiae Plantarum, 01.06.2018.

Resultado de la investigación: Contribución a una revistaArtículo

TY - JOUR

T1 - Double herbicide-resistant biotypes of wild oat (Avena fatua) display characteristic metabolic fingerprints before and after applying ACCase- and ALS-inhibitors

AU - Torres-García, Jesús R.

AU - Tafoya-Razo, J. Antonio

AU - Velázquez-Márquez, Sabina

AU - Tiessen, Axel

PY - 2018/6/1

Y1 - 2018/6/1

N2 - © 2018, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków. Plant herbicides inhibit specific enzymes of biosynthetic metabolism, such as acetyl-coenzyme A carboxylase (ACCase) and acetolactate synthase (ALS). Herbicide resistance can be caused by point mutations at the binding domains, catalytic sites and other regions within multimeric enzymes. Direct-injection electrospray mass spectrometry was used for high-throughput metabolic fingerprinting for finding significant differences among biotypes in response to herbicide application. A Mexican biotype of wild oat (Avena fatua) that displays multiple resistances to ACCase- and ALS-inhibiting herbicides was characterized. The dose–response test showed that the double-resistant biotype had a resistance index of 3.58 for pinoxaden and 3.53 for mesosulfuron-methyl. Resistance was accompanied by characteristic mutations at the site of action: an I-1781-L substitution occurred in the ACCase enzyme and an S-653-N mutation was identified within the ALS enzyme. Other mutations were also detected in the genes of the Mexican biotypes. The ionomic fingerprint showed that the multiple-resistant biotype had a markedly different metabolic pattern under control conditions and that this difference was accentuated after herbicide treatment. This demonstrates that single changes of amino acid sequences can produce several holistic modifications in the metabolism of resistant plants compared to susceptible plants. We conclude that in addition to genetic resistance, additional mechanisms of metabolic adaptation and detoxification can occur in multiple-resistant weed plants.

AB - © 2018, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków. Plant herbicides inhibit specific enzymes of biosynthetic metabolism, such as acetyl-coenzyme A carboxylase (ACCase) and acetolactate synthase (ALS). Herbicide resistance can be caused by point mutations at the binding domains, catalytic sites and other regions within multimeric enzymes. Direct-injection electrospray mass spectrometry was used for high-throughput metabolic fingerprinting for finding significant differences among biotypes in response to herbicide application. A Mexican biotype of wild oat (Avena fatua) that displays multiple resistances to ACCase- and ALS-inhibiting herbicides was characterized. The dose–response test showed that the double-resistant biotype had a resistance index of 3.58 for pinoxaden and 3.53 for mesosulfuron-methyl. Resistance was accompanied by characteristic mutations at the site of action: an I-1781-L substitution occurred in the ACCase enzyme and an S-653-N mutation was identified within the ALS enzyme. Other mutations were also detected in the genes of the Mexican biotypes. The ionomic fingerprint showed that the multiple-resistant biotype had a markedly different metabolic pattern under control conditions and that this difference was accentuated after herbicide treatment. This demonstrates that single changes of amino acid sequences can produce several holistic modifications in the metabolism of resistant plants compared to susceptible plants. We conclude that in addition to genetic resistance, additional mechanisms of metabolic adaptation and detoxification can occur in multiple-resistant weed plants.

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