TY - JOUR
T1 - Differential expression of dnaA and dosR genes among members of the Mycobacterium tuberculosis complex under oxic and hypoxic conditions
AU - Badillo-López, Claudia
AU - González-Mejía, Arturo
AU - Helguera-Repetto, A. Cecilia
AU - Salas-Rangel, Laura P.
AU - Rivera-Gutiérrez, Sandra
AU - Cerna-Cortés, Jorge F.
AU - González-y-Merchand, Jorge A.
PY - 2010/3
Y1 - 2010/3
N2 - Major differences regarding the pathology and host immune response of the Beijing and Canettii genotypes of Mycobacterium tuberculosis have been reported; however, studies on the genetic expression of these genotypes during in vitro dormancy are scarce. This study examined the expression of five cell-cycle-related genes and two dormancy-related genes in M. canettii, M. tuberculosis H37Rv, and M. tuberculosis Beijing during the Wayne model of dormancy. The results showed that under hypoxic conditions the three tuberculosis genotypes were able to transcribe genes involved in DNA replication and cellular division. In addition, dosR was found to be up-regulated in M. tuberculosis Beijing during the exponential growth phase but down-regulated under hypoxic conditions. In this genotype, the replication-related gene dnaA was also strongly down-regulated. These latter two findings suggest that, compared to M. tuberculosis H37Rv and M. canettii, the Beijing genotype has a lower capacity to synthesize dosR, hspX, and dnaA mRNAs during in vitro dormancy.
AB - Major differences regarding the pathology and host immune response of the Beijing and Canettii genotypes of Mycobacterium tuberculosis have been reported; however, studies on the genetic expression of these genotypes during in vitro dormancy are scarce. This study examined the expression of five cell-cycle-related genes and two dormancy-related genes in M. canettii, M. tuberculosis H37Rv, and M. tuberculosis Beijing during the Wayne model of dormancy. The results showed that under hypoxic conditions the three tuberculosis genotypes were able to transcribe genes involved in DNA replication and cellular division. In addition, dosR was found to be up-regulated in M. tuberculosis Beijing during the exponential growth phase but down-regulated under hypoxic conditions. In this genotype, the replication-related gene dnaA was also strongly down-regulated. These latter two findings suggest that, compared to M. tuberculosis H37Rv and M. canettii, the Beijing genotype has a lower capacity to synthesize dosR, hspX, and dnaA mRNAs during in vitro dormancy.
KW - Cell cycle
KW - Differential gene expression
KW - Hypoxia
KW - Mycobacterium tuberculosis complex
UR - http://www.scopus.com/inward/record.url?scp=79251567991&partnerID=8YFLogxK
U2 - 10.2436/20.1501.01.106
DO - 10.2436/20.1501.01.106
M3 - Artículo
SN - 1139-6709
VL - 13
SP - 9
EP - 13
JO - International Microbiology
JF - International Microbiology
IS - 1
ER -