TY - JOUR
T1 - Different expression of α and β mitochondrial estrogen receptors in the aging rat brain
T2 - Interaction with respiratory complex V
AU - Álvarez-Delgado, Carolina
AU - Mendoza-Rodríguez, C. Adriana
AU - Picazo, Ofir
AU - Cerbón, Marco
N1 - Funding Information:
This work was supported by grants from PAPIIT, PAIP, UNAM, CONACyT, COFAA and SIP-IPN. CAD has a fellowship from CONACyT.
PY - 2010/8
Y1 - 2010/8
N2 - Recent evidence suggests that hormonal effects on mitochondria could be mediated by mitochondrial estrogen receptors (mtERs). These receptors are new candidates for the beneficial estrogenic effects on mitochondria in different physiological conditions. The aim of this investigation was to study mtER expression during brain aging. We analyzed mtERα and mtERβ expression in cortical, hippocampal and hypothalamic mitochondria of young adult (3. months) and aged (18. months) female Wistar rats by Western blot. In addition, we explored the interaction of mtERβ with respiratory complex V by using coimmunoprecipitation assays. The results show that mtERα and mtERβ are present in young and aged brain mitochondria. We also demonstrate that mtERs are expressed as variants and have a brain region specific distribution. The predominant mtER variants detected were of 61 and 55. KDa for mtERα and of 63 and 52. KDa for mtERβ. However, we did not observe differences in the mtERα or β content between the two age groups studied. Additionally, we show that mtERβ interacts with complex V.The overall results demonstrate that there is a differential expression of mtERα and mtERβ variants in different brain areas, indicating that they may participate in different functions in the brain during aging.
AB - Recent evidence suggests that hormonal effects on mitochondria could be mediated by mitochondrial estrogen receptors (mtERs). These receptors are new candidates for the beneficial estrogenic effects on mitochondria in different physiological conditions. The aim of this investigation was to study mtER expression during brain aging. We analyzed mtERα and mtERβ expression in cortical, hippocampal and hypothalamic mitochondria of young adult (3. months) and aged (18. months) female Wistar rats by Western blot. In addition, we explored the interaction of mtERβ with respiratory complex V by using coimmunoprecipitation assays. The results show that mtERα and mtERβ are present in young and aged brain mitochondria. We also demonstrate that mtERs are expressed as variants and have a brain region specific distribution. The predominant mtER variants detected were of 61 and 55. KDa for mtERα and of 63 and 52. KDa for mtERβ. However, we did not observe differences in the mtERα or β content between the two age groups studied. Additionally, we show that mtERβ interacts with complex V.The overall results demonstrate that there is a differential expression of mtERα and mtERβ variants in different brain areas, indicating that they may participate in different functions in the brain during aging.
KW - Aging
KW - Complex V
KW - Mitochondrial estrogen receptors
KW - MtERα
KW - MtERβ
KW - Neuroprotection
KW - Rat brain
UR - http://www.scopus.com/inward/record.url?scp=77953360296&partnerID=8YFLogxK
U2 - 10.1016/j.exger.2010.01.015
DO - 10.1016/j.exger.2010.01.015
M3 - Artículo
C2 - 20096765
SN - 0531-5565
VL - 45
SP - 580
EP - 585
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 7-8
ER -