TY - JOUR
T1 - Development of ultra-low friction coefficient films and their effect on the biocompatibility of biomedical steel
AU - Chino-Ulloa, Alexis
AU - Hernandes-Alejandro, Mario
AU - Castrejón-Flores, José L.
AU - Cabrera-González, Moisés
AU - Torres-Avila, Itzel P.
AU - Velázquez, Julio C.
AU - Ruiz-Trabolsi, Pablo A.
AU - Hernández-Sánchez, Enrique
N1 - Publisher Copyright:
© 2019 The Japan Institute of Metals and Materials
PY - 2019
Y1 - 2019
N2 - This study evaluates the effect of a self-lubricant film with ultra-low friction coefficient on the biocompatibility of the AISI 316L. The samples were thermochemically treated to generate a hard surface layer of iron borides followed by a secondary process. The purpose of the secondary process was to generate a self-lubricant film of boric acid. The ultra-low friction coefficient of boric acid films was estimated in 0.02 compared to 0.2 and 0.8 for borided and as-received AISI 316L steel respectively. The effect of the boric acid films on the steel's biocompatibility was evaluated by the culture of Madin-Darby canine kidney II (MDCK II) cells with exposure times of 0, 4, 8, 12, 24, 48 and 72 h. The In vitro tests revealed the absence of cytotoxic effect by the direct exposure of the cells to the samples. Additionally, the presence of signs early apoptosis by the determination of the expression of the cysteinyl proteases termed caspase-3 and -9 was determined. Their presence was assessed by indirect immunofluorescence in cells in direct contact with the samples. No significant expression of the proteolytic enzymes was detected. All these results suggest that the surface film of boric acid does not induce any cytotoxic effect and does not inhibit the cell-cell adhesion.
AB - This study evaluates the effect of a self-lubricant film with ultra-low friction coefficient on the biocompatibility of the AISI 316L. The samples were thermochemically treated to generate a hard surface layer of iron borides followed by a secondary process. The purpose of the secondary process was to generate a self-lubricant film of boric acid. The ultra-low friction coefficient of boric acid films was estimated in 0.02 compared to 0.2 and 0.8 for borided and as-received AISI 316L steel respectively. The effect of the boric acid films on the steel's biocompatibility was evaluated by the culture of Madin-Darby canine kidney II (MDCK II) cells with exposure times of 0, 4, 8, 12, 24, 48 and 72 h. The In vitro tests revealed the absence of cytotoxic effect by the direct exposure of the cells to the samples. Additionally, the presence of signs early apoptosis by the determination of the expression of the cysteinyl proteases termed caspase-3 and -9 was determined. Their presence was assessed by indirect immunofluorescence in cells in direct contact with the samples. No significant expression of the proteolytic enzymes was detected. All these results suggest that the surface film of boric acid does not induce any cytotoxic effect and does not inhibit the cell-cell adhesion.
KW - Biocompatibility
KW - Boric acid
KW - Cytotoxicity
KW - Solid lubricant
KW - Ultra-low friction coefficient
UR - http://www.scopus.com/inward/record.url?scp=85069712342&partnerID=8YFLogxK
U2 - 10.2320/matertrans.M2019035
DO - 10.2320/matertrans.M2019035
M3 - Artículo
AN - SCOPUS:85069712342
SN - 1345-9678
VL - 60
SP - 1605
EP - 1613
JO - Materials Transactions
JF - Materials Transactions
IS - 8
ER -