TY - JOUR
T1 - Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis
AU - Hernandez-Cruz, E.
AU - González-Cabriales, J. J.
AU - Ordaz-Pichardo, C.
AU - De La Cruz-Hernández, N. I.
AU - Flores-Gutiérrez, G. H.
PY - 2009/12
Y1 - 2009/12
N2 - An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P<0.05, kappa=0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis.
AB - An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P<0.05, kappa=0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis.
UR - http://www.scopus.com/inward/record.url?scp=77949656390&partnerID=8YFLogxK
U2 - 10.1017/S0022149X09270866
DO - 10.1017/S0022149X09270866
M3 - Artículo
SN - 0022-149X
VL - 83
SP - 333
EP - 337
JO - Journal of Helminthology
JF - Journal of Helminthology
IS - 4
ER -