TY - JOUR
T1 - Determinación y análisis del perfil proteico de diferentes factores de transferencia
AU - Guidos-Fogelbach, Guillermo Arturo
AU - Paredes-Aguilar, Jorge Antonio
AU - Colín-Martínez, Nayeli Montserrat
AU - Rojo-Gutiérrez, María Isabel
AU - López-Hidalgo, Marisol
AU - Reyes-López, César Augusto Sandino
N1 - Publisher Copyright:
© 2016 Nieto Editores. All rights reserved.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Background: The transfer factor (TF) is the dialyzable extract of leukocytes with cellular immunity transfer properties. Its use has spread in the treatment of a wide range of immunologic, infectious, and even oncological diseases. However, important aspects in their protein profile, component concentrations, and a well-defined action mechanism are not completely unknown. Objectives: To analyze the protein profiles of different transfer factors marketed in Mexico. Methods: 6 TF marketed in Mexico were obtained and analyzed, quantifying protein with thaze Bradford method, by high-performance liquid chromatography (HPLC), and polyacrylamide gel electrophoresis (SDS-PAGE). All samples were analyzed in duplicate. Results: The total protein concentrations of all TF analyzed are less than 0.2 mg/mL. The chromatographic profiles showed differences in some TF. The protein concentration was 6 to almost one thousand times lower compared to reports by some manufacturers. Conclusion: Almost all transfer factors marketed in Mexico lack a labeling and health record that meets the official standards.
AB - Background: The transfer factor (TF) is the dialyzable extract of leukocytes with cellular immunity transfer properties. Its use has spread in the treatment of a wide range of immunologic, infectious, and even oncological diseases. However, important aspects in their protein profile, component concentrations, and a well-defined action mechanism are not completely unknown. Objectives: To analyze the protein profiles of different transfer factors marketed in Mexico. Methods: 6 TF marketed in Mexico were obtained and analyzed, quantifying protein with thaze Bradford method, by high-performance liquid chromatography (HPLC), and polyacrylamide gel electrophoresis (SDS-PAGE). All samples were analyzed in duplicate. Results: The total protein concentrations of all TF analyzed are less than 0.2 mg/mL. The chromatographic profiles showed differences in some TF. The protein concentration was 6 to almost one thousand times lower compared to reports by some manufacturers. Conclusion: Almost all transfer factors marketed in Mexico lack a labeling and health record that meets the official standards.
KW - Protein analysis
KW - Transfer factor
UR - http://www.scopus.com/inward/record.url?scp=85054383118&partnerID=8YFLogxK
U2 - 10.29262/ram.v63i4.190
DO - 10.29262/ram.v63i4.190
M3 - Artículo
SN - 0002-5151
VL - 63
SP - 365
EP - 372
JO - Revista Alergia Mexico
JF - Revista Alergia Mexico
IS - 4
ER -