TY - JOUR
T1 - Detection of white spot syndrome virus in filtered shrimp-farm water fractions and experimental evaluation of its infectivity in Penaeus (Litopenaeus) vannamei
AU - Esparza-Leal, Héctor M.
AU - Escobedo-Bonilla, César M.
AU - Casillas-Hernández, Ramón
AU - Álvarez-Ruíz, Píndaro
AU - Portillo-Clark, Guillermo
AU - Valerio-García, Roberto C.
AU - Hernández-López, Jorge
AU - Méndez-Lozano, Jesús
AU - Vibanco-Pérez, Norberto
AU - Magallón-Barajas, Francisco J.
N1 - Funding Information:
This study is part of the Consortium for Aquaculture Innovation and Competitivity funded by Consejo Nacional de Ciencia y Tecnología (grant I0004-2006-02-60030 to R.P.E.), Comisión Nacional Acuacultura y Pesca-Sonora State Government (grant to J.H.L.), and Comités de Sanidad Acuícola from Sinaloa (CESASIN), Sonora (COSAES), Nayarit (CESANAY), and Baja California Sur (CSABCS). H.M.E.L. received a doctoral fellowship from CONACYT (grant 208764) and Instituto Politécnico Nacional (license 352, PL-151). Thanks to N. Mañon-Ríos and J.L. Romero-Romero for their help during this study and to the personnel from ‘Acualarvas’ hatchery and ‘Loma de Las Pitayas' shrimp farm.
PY - 2009/7/1
Y1 - 2009/7/1
N2 - White spot syndrome virus (WSSV) may spread through water to neighbor ponds or farms. Routine water exchange and wastewater released during white spot disease (WSD)-emergency harvests may preserve WSSV in shrimp farming areas. To test this hypothesis, on-site experiments were performed in a WSSV-affected farm in Guasave, Sinaloa, Mexico. Plankton and shrimp hemolymph were collected from 12 ponds during a WSD outbreak. PCR analyses showed that 72% of the hemolymph pools (26 out of 36) were WSSV-positive. In contrast, only 14% (4 of 28) plankton samples (filtered through 10 and 0.45 μm) from three ponds (2, 7 and 10) were WSSV-positive. Plankton from pond 9 was WSSV-negative, but 14 days later, shrimp began to die. At this point, a differential filtration experiment was performed in pond 9. WSSV-positive samples were only found in three fractions [particulate fraction (PF) 1 μm and liquid fractions (LF) < 100 and < 40 μm]. Both LFs and PFs were used for in situ infectivity assays by water-borne routes in WSSV-negative whiteleg shrimp Penaeus (Litopenaeus) vannamei. Some shrimp exposed to different PFs and LFs (100 μm to > 0.65 μm) became WSSV-positive. Results indicate that water fractions between 100 and 0.65 μm induced WSSV infection to shrimp. Results showed that pond water and/or particulate fractions are vehicles for WSSV dispersion via virus suspended in water, attached to microalgae, or carried by zooplankton.
AB - White spot syndrome virus (WSSV) may spread through water to neighbor ponds or farms. Routine water exchange and wastewater released during white spot disease (WSD)-emergency harvests may preserve WSSV in shrimp farming areas. To test this hypothesis, on-site experiments were performed in a WSSV-affected farm in Guasave, Sinaloa, Mexico. Plankton and shrimp hemolymph were collected from 12 ponds during a WSD outbreak. PCR analyses showed that 72% of the hemolymph pools (26 out of 36) were WSSV-positive. In contrast, only 14% (4 of 28) plankton samples (filtered through 10 and 0.45 μm) from three ponds (2, 7 and 10) were WSSV-positive. Plankton from pond 9 was WSSV-negative, but 14 days later, shrimp began to die. At this point, a differential filtration experiment was performed in pond 9. WSSV-positive samples were only found in three fractions [particulate fraction (PF) 1 μm and liquid fractions (LF) < 100 and < 40 μm]. Both LFs and PFs were used for in situ infectivity assays by water-borne routes in WSSV-negative whiteleg shrimp Penaeus (Litopenaeus) vannamei. Some shrimp exposed to different PFs and LFs (100 μm to > 0.65 μm) became WSSV-positive. Results indicate that water fractions between 100 and 0.65 μm induced WSSV infection to shrimp. Results showed that pond water and/or particulate fractions are vehicles for WSSV dispersion via virus suspended in water, attached to microalgae, or carried by zooplankton.
KW - Differential filtration
KW - Infectivity assay
KW - Penaeus (Litopenaeus) vannamei
KW - Pond water
KW - Shrimp farm
KW - WSSV
UR - http://www.scopus.com/inward/record.url?scp=65649099163&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2009.03.021
DO - 10.1016/j.aquaculture.2009.03.021
M3 - Artículo
SN - 0044-8486
VL - 292
SP - 16
EP - 22
JO - Aquaculture
JF - Aquaculture
IS - 1-2
ER -