Detection of Salmonella enterica on silicon substrates biofunctionalized with anti-Salmonella IgG, analyzed by FTIR spectroscopy

Título traducido de la contribución: Detección de Salmonella enterica sobre sustratos de silicio biofuncionalizados con IgG anti-Salmonella, analizados por espectroscopía FTIR

F. J. Gómez-Montaño, A. Orduña-Díaz, M. C.G. Avelino-Flores, F. Avelino-Flores, F. Ramos-Collazo, C. Reyes-Betanzo, V. López-Gayou

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

5 Citas (Scopus)

Resumen

Salmonella enterica was detected at a concentration of 500 CFU/mL. For this, three substrates were used (crystalline and amorphous silicon, hydrogenated amorphous silicon carbide) with an area of 25 mm2, functionalized by the self-assembled monolayers (SAM) technique. Characteristic functional groups of SAMs were observed (-NH2, -C=O, Si-O-Si, Amide I, Amide II) by the Fourier transform infrared spectroscopy (FTIR) technique. Anti-Salmonella IgG antibodies were generated. To determine the secondary structure of proteins, second derivative of each FTIR detection spectrum was generated, observing modifications in the zone between 1600-1700 cm-1, which corresponds to the region of proteins, specifically to structures of β- sheets and β-turns, as a result, substrates or platforms of crystalline silicon and hydrogenated amorphous silicon carbide showed band frequencies with higher intensity, being this attributed to a better sensitivity, which are proposed for applications in the development of biosensors for the monitoring of microbial quality in foods.

Título traducido de la contribuciónDetección de Salmonella enterica sobre sustratos de silicio biofuncionalizados con IgG anti-Salmonella, analizados por espectroscopía FTIR
Idioma originalInglés
Páginas (desde-hasta)1175-1185
Número de páginas11
PublicaciónRevista Mexicana de Ingeniera Quimica
Volumen19
N.º3
DOI
EstadoPublicada - 1 sep. 2020

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