TY - JOUR
T1 - Detection of Salmonella enterica on silicon substrates biofunctionalized with anti-Salmonella IgG, analyzed by FTIR spectroscopy
AU - Gómez-Montaño, F. J.
AU - Orduña-Díaz, A.
AU - Avelino-Flores, M. C.G.
AU - Avelino-Flores, F.
AU - Ramos-Collazo, F.
AU - Reyes-Betanzo, C.
AU - López-Gayou, V.
N1 - Publisher Copyright:
© 2020, Universidad Autonoma Metropolitana Iztapalapa. All rights reserved.
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Salmonella enterica was detected at a concentration of 500 CFU/mL. For this, three substrates were used (crystalline and amorphous silicon, hydrogenated amorphous silicon carbide) with an area of 25 mm2, functionalized by the self-assembled monolayers (SAM) technique. Characteristic functional groups of SAMs were observed (-NH2, -C=O, Si-O-Si, Amide I, Amide II) by the Fourier transform infrared spectroscopy (FTIR) technique. Anti-Salmonella IgG antibodies were generated. To determine the secondary structure of proteins, second derivative of each FTIR detection spectrum was generated, observing modifications in the zone between 1600-1700 cm-1, which corresponds to the region of proteins, specifically to structures of β- sheets and β-turns, as a result, substrates or platforms of crystalline silicon and hydrogenated amorphous silicon carbide showed band frequencies with higher intensity, being this attributed to a better sensitivity, which are proposed for applications in the development of biosensors for the monitoring of microbial quality in foods.
AB - Salmonella enterica was detected at a concentration of 500 CFU/mL. For this, three substrates were used (crystalline and amorphous silicon, hydrogenated amorphous silicon carbide) with an area of 25 mm2, functionalized by the self-assembled monolayers (SAM) technique. Characteristic functional groups of SAMs were observed (-NH2, -C=O, Si-O-Si, Amide I, Amide II) by the Fourier transform infrared spectroscopy (FTIR) technique. Anti-Salmonella IgG antibodies were generated. To determine the secondary structure of proteins, second derivative of each FTIR detection spectrum was generated, observing modifications in the zone between 1600-1700 cm-1, which corresponds to the region of proteins, specifically to structures of β- sheets and β-turns, as a result, substrates or platforms of crystalline silicon and hydrogenated amorphous silicon carbide showed band frequencies with higher intensity, being this attributed to a better sensitivity, which are proposed for applications in the development of biosensors for the monitoring of microbial quality in foods.
KW - FTIR
KW - IgG
KW - Salmonella
KW - Silicon
KW - biosensor
UR - http://www.scopus.com/inward/record.url?scp=85086264019&partnerID=8YFLogxK
U2 - 10.24275/rmiq/Bio993
DO - 10.24275/rmiq/Bio993
M3 - Artículo
SN - 1665-2738
VL - 19
SP - 1175
EP - 1185
JO - Revista Mexicana de Ingeniera Quimica
JF - Revista Mexicana de Ingeniera Quimica
IS - 3
ER -