TY - GEN
T1 - Detection of leukemia with blood samples using raman spectroscopy and multivariate analysis
AU - Martínez-Espinosa, J. C.
AU - González-Solís, J. L.
AU - Miranda-Beltrán, M. L.
AU - Soria-Fregoso, C.
AU - Medina-Valtierra, J.
AU - Frausto-Reyes, C.
PY - 2009
Y1 - 2009
N2 - The use of Raman spectroscopy to analyze blood biochemistry and hence distinguish between normal and abnormal blood was investigated. Blood samples were obtained from 6 patients who were clinically diagnosed with leukemia and 6 healthy volunteers. The imprint was put under the microscope and several points were chosen for Raman measurement. All the spectra were collected by a confocal Raman micro-spectroscopy (Renishaw) with a NIR 830 nm laser. It is shown that the serum samples from patients with leukemia and from the control group can be discriminated when the multivariate statistical methods of principal component analysis (PCA) and linear discriminated analysis (LDA) are applied to their Raman spectra. The ratios of some band intensities were analyzed and some band ratios were significant and corresponded to proteins, phospholipids, and polysaccharides. The preliminary results suggest that Raman Spectroscopy could be a new technique to study the degree of damage to the bone marrow using just blood samples instead of biopsies, treatment very painful for patients.
AB - The use of Raman spectroscopy to analyze blood biochemistry and hence distinguish between normal and abnormal blood was investigated. Blood samples were obtained from 6 patients who were clinically diagnosed with leukemia and 6 healthy volunteers. The imprint was put under the microscope and several points were chosen for Raman measurement. All the spectra were collected by a confocal Raman micro-spectroscopy (Renishaw) with a NIR 830 nm laser. It is shown that the serum samples from patients with leukemia and from the control group can be discriminated when the multivariate statistical methods of principal component analysis (PCA) and linear discriminated analysis (LDA) are applied to their Raman spectra. The ratios of some band intensities were analyzed and some band ratios were significant and corresponded to proteins, phospholipids, and polysaccharides. The preliminary results suggest that Raman Spectroscopy could be a new technique to study the degree of damage to the bone marrow using just blood samples instead of biopsies, treatment very painful for patients.
KW - Bone marrow
KW - Leukemia
KW - Multivariate
KW - Raman spectroscopy
KW - Serum
UR - http://www.scopus.com/inward/record.url?scp=70450200613&partnerID=8YFLogxK
U2 - 10.1063/1.3175637
DO - 10.1063/1.3175637
M3 - Contribución a la conferencia
AN - SCOPUS:70450200613
SN - 9780735406797
T3 - AIP Conference Proceedings
SP - 99
EP - 103
BT - Laser Florence 2008 - A Bridge to the Laser Medicine World
T2 - International Laser Medicine Congress, Laser Florence 2008: A Bridge To The Laser Medicine World
Y2 - 31 October 2008 through 1 November 2008
ER -