Efecto citotónico y citotóxico de la toxina colérica en células Vero y su relación con PCR.

M. G. Rodríguez-Angeles, S. Giono-Cerezo, J. L. Valdespino-Gómez

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

4 Citas (Scopus)

Resumen

We studied 40 Vibrio cholerae strains: 16 from stool, 16 from sewage and 8 from food. The serotypes were Inaba in 21 strains, 8 Ogawa strains and 11 V. cholerae non-O1. PCR was made with ctx2 and ctx3 primers with 25 cycles of temperature: 1 min at 94 degrees C, 1 min at 60 degrees C and 1 min at 72 degrees C. 24 V. cholerae strains were positive: 18/24 Inaba y 6/24 Ogawa. PCR was negative for 16 strains: 3 Inaba serotype, 2 Ogawa y 11 V. cholerae non-O1. In Vero culture cells 18 strains were cytotonic, 21 cytotoxic and 1 strain was negative. ELISA was positive for 11 strains with PCR positive. The PCR sensitivity was 95.83% compared with culture cells. V. cholerae O1 produced cytotoxic effect on Vero culture cells, maybe related to ACE factor. Colony blot was made with a specific probe labeled with digoxigenin and it could detect 4 Vibrio cholerae toxigenic strains with PCR negative. All V. cholerae Non O1 strains were PCR negative.

Título traducido de la contribuciónCytotonic and cytotoxic effect of cholera toxin on Vero cells and its relation to PCR
Idioma originalEspañol
Páginas (desde-hasta)263-271
Número de páginas9
PublicaciónRevista latinoamericana de microbiologia
Volumen36
N.º4
EstadoPublicada - 1994
Publicado de forma externa

Huella

Profundice en los temas de investigación de 'Efecto citotónico y citotóxico de la toxina colérica en células Vero y su relación con PCR.'. En conjunto forman una huella única.

Citar esto