TY - JOUR
T1 - Compound distribution, structural analysis and nanomechanical properties of nanofibers loaded with high-oleic palm oil nanoemulsions for packaging application
AU - Ricaurte, Leidy
AU - Perea-Flores, María de Jesús
AU - Méndez-Méndez, Juan Vicente
AU - Santagapita, Patricio Román
AU - Quintanilla-Carvajal, Maria Ximena
N1 - Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/3/5
Y1 - 2022/3/5
N2 - The effect of the HOPO concentration on the morphology, fiber diameter, oil load and distribution, protein structure, protein-oil colocalization, and nanomechanical properties of the NFs were evaluated. We obtained smooth and defect-free NFs with diameters in range of 77–94 nm. Fourier-transform infrared spectroscopy was used as a reliable method to confirm oil load and protein structure after electrospinning. β-turn, β-sheet, α-helix, and triple helical structures were quantified. Confocal laser scanning microscopy was used to analyze colocalization, and results revealed a high distribution of NE droplets along the NFs and high oil-protein colocalization (overlap coefficient of ~ 0.63). The nanomechanical properties of the NE-loaded NFs demonstrated that at higher HOPO concentrations, the elastic modulus decreased. Likewise, homogeneity and synergy between compounds were observed. The adhesion properties of NE-loaded NFs were associated with their sticky surface, which was attributed to presence of HOPO. The NE-loaded NFs presented suitable physical properties for edible packaging.
AB - The effect of the HOPO concentration on the morphology, fiber diameter, oil load and distribution, protein structure, protein-oil colocalization, and nanomechanical properties of the NFs were evaluated. We obtained smooth and defect-free NFs with diameters in range of 77–94 nm. Fourier-transform infrared spectroscopy was used as a reliable method to confirm oil load and protein structure after electrospinning. β-turn, β-sheet, α-helix, and triple helical structures were quantified. Confocal laser scanning microscopy was used to analyze colocalization, and results revealed a high distribution of NE droplets along the NFs and high oil-protein colocalization (overlap coefficient of ~ 0.63). The nanomechanical properties of the NE-loaded NFs demonstrated that at higher HOPO concentrations, the elastic modulus decreased. Likewise, homogeneity and synergy between compounds were observed. The adhesion properties of NE-loaded NFs were associated with their sticky surface, which was attributed to presence of HOPO. The NE-loaded NFs presented suitable physical properties for edible packaging.
KW - Biofilm
KW - Colocalization
KW - Electrospinning
KW - Mechanical properties
KW - Protein structure
UR - http://www.scopus.com/inward/record.url?scp=85122381773&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfa.2021.128148
DO - 10.1016/j.colsurfa.2021.128148
M3 - Artículo
AN - SCOPUS:85122381773
SN - 0927-7757
VL - 636
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
M1 - 128148
ER -