TY - JOUR
T1 - Comparing RAS with and without biofloc
T2 - Transcriptional response of immune-related genes in litopenaeus vannamei post-larvae
AU - Soto-Alcalá, Jorge
AU - Álvarez-Ruiz, Píndaro
AU - Audelo-Naranjo, Juan M.
AU - Esparza-Leal, Héctor M.
AU - Luis-Villaseñor, Irasema E.
AU - Estrada-Godínez, José A.
AU - Luna-González, Antonio
AU - Gámez-Jiménez, Carina
AU - Diarte-Plata, Genaro
N1 - Publisher Copyright:
© 2020, Universidad de Antioquia. All rights reserved.
PY - 2020/1
Y1 - 2020/1
N2 - Background: Shrimp farming is evolving from semi-intensive to hyper-intensive systems with biofloc technology and water recirculation systems. Objective: To evaluate the transcriptional response promoted by biofloc on shrimp (Litopenaeus vannamei) under a recirculating aquaculture system (RAS). Methods: Quantitative real-time RT-PCR was used to monitor seven key genes related to the immune system in shrimp post-larvae, reared in a RAS with and without biofloc (BF and no-BF). In addition, we present for the first time nucleotide sequences of ADP-ribosylation factor 4 (LvArf4) from Litopenaeus vannamei. Results: Transcripts for penaeidin3 (Pen3), penaeidin4 (Pen4), crustin, and Toll receptor (LvToll) genes were up-regulated between 3 and 24 h in both systems, and tumor necrosis factor receptor-associated factor 6 (TRAF6) in no-BF as an early response. Regarding differential expression between treatments, 13 occurrences were encountered. Nine that were higher in BF than in no-BF and four higher in no-BF than in BF. In some sample times, expression of Pen3, crustin, LvToll, TRAF6, IMD, and LvArf4 was higher in BF than in no-BF and in others, expression of Pen3, Pen4, and TRAF6 was higher in no-BF than in BF. Conclusions: BF modulates the transcription of genes related to the immune response in shrimp as an early response. However, the RAS with no-BF promotes a similar response.
AB - Background: Shrimp farming is evolving from semi-intensive to hyper-intensive systems with biofloc technology and water recirculation systems. Objective: To evaluate the transcriptional response promoted by biofloc on shrimp (Litopenaeus vannamei) under a recirculating aquaculture system (RAS). Methods: Quantitative real-time RT-PCR was used to monitor seven key genes related to the immune system in shrimp post-larvae, reared in a RAS with and without biofloc (BF and no-BF). In addition, we present for the first time nucleotide sequences of ADP-ribosylation factor 4 (LvArf4) from Litopenaeus vannamei. Results: Transcripts for penaeidin3 (Pen3), penaeidin4 (Pen4), crustin, and Toll receptor (LvToll) genes were up-regulated between 3 and 24 h in both systems, and tumor necrosis factor receptor-associated factor 6 (TRAF6) in no-BF as an early response. Regarding differential expression between treatments, 13 occurrences were encountered. Nine that were higher in BF than in no-BF and four higher in no-BF than in BF. In some sample times, expression of Pen3, crustin, LvToll, TRAF6, IMD, and LvArf4 was higher in BF than in no-BF and in others, expression of Pen3, Pen4, and TRAF6 was higher in no-BF than in BF. Conclusions: BF modulates the transcription of genes related to the immune response in shrimp as an early response. However, the RAS with no-BF promotes a similar response.
KW - Antimicrobial peptide
KW - Aquaculture
KW - Biofloc
KW - Gene expression
KW - Penaeidin
KW - RAS
KW - Shrimp
KW - Shrimp immune system
UR - http://www.scopus.com/inward/record.url?scp=85079138168&partnerID=8YFLogxK
U2 - 10.17533/udea.rccp.v33n1a03
DO - 10.17533/udea.rccp.v33n1a03
M3 - Artículo
AN - SCOPUS:85079138168
SN - 0120-0690
VL - 33
SP - 32
EP - 43
JO - Revista Colombiana de Ciencias Pecuarias
JF - Revista Colombiana de Ciencias Pecuarias
IS - 1
ER -