TY - JOUR
T1 - Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons
AU - Blancarte–Lagunas, Manalli Itzahaya
AU - Castro-Escarpulli, Graciela
AU - Navarro-Ocaña, Armando
AU - Ibáñez-Cervantes, Gabriela
AU - Marquez-Valdelamar, Laura Margarita
AU - Hernández-Carrillo, Jose Misael
AU - Salazar-Salinas, Juana
AU - Mendoza-Vásquez, Omar Fernando
AU - Dámazo-Hernández, Gabriel
AU - Sosa-Hernández, Oscar
AU - León-García, Gregorio
AU - Cureño-Díaz, Monica Alethia
AU - Bello–López, Juan Manuel
N1 - Publisher Copyright:
Copyright © 2020 Blancarte–Lagunas et al.
PY - 2020
Y1 - 2020
N2 - Introduction: Antimicrobial resistance in Escherichia coli, one of the causal agents of aerobic vaginitis, leads to the persistence of the infection. The investigation of integrons acquires relevance, since they are elements that are responsible for the acquisition of resistance to antibiotics. The aim of this work was to describe the structural diversity of class 1 integrons in virulent and commensal strains of E. coli isolated from patients with vaginal infection. Methodology: Ninety-two strains of E. coli were isolated from patients with aerobic vaginitis. Resistance profile against 19 antibiotics and class 1 integrons were detected by PCR. The identity and arrangement of cassettes was determined by sequencing. ERIC-PCR assays were carried out in strains with identical arrays. Finally, genotyping by Clermont algorithm and serotyping were performed. Seventeen strains showed integrons located in plasmids. Results: Ten different gene cassette arrays were identified in 30 strains of E. coli. Cassettes corresponding to genes coding for adenylyltransferases (aadA), dihydrofolate reductases (dfrA), and oxacillinases (blaOXA) were detected. Array dfrA17-aadA5 was predominantly prevalent over the other arrays identified. Phylogenetic group A was the most predominant, followed by group B2 and D. Conclusions: This study demonstrates the presence of E. coli of vaginal origin carrying class 1 integrons, which are main genetic elements of capture of resistance genes, with the possibility of capturing new resistance cassettes. These evidences should serve for the modification of protocols in the diagnosis and treatment of aerobic vaginitis, and the development of policies for the rational use of antimicrobials.
AB - Introduction: Antimicrobial resistance in Escherichia coli, one of the causal agents of aerobic vaginitis, leads to the persistence of the infection. The investigation of integrons acquires relevance, since they are elements that are responsible for the acquisition of resistance to antibiotics. The aim of this work was to describe the structural diversity of class 1 integrons in virulent and commensal strains of E. coli isolated from patients with vaginal infection. Methodology: Ninety-two strains of E. coli were isolated from patients with aerobic vaginitis. Resistance profile against 19 antibiotics and class 1 integrons were detected by PCR. The identity and arrangement of cassettes was determined by sequencing. ERIC-PCR assays were carried out in strains with identical arrays. Finally, genotyping by Clermont algorithm and serotyping were performed. Seventeen strains showed integrons located in plasmids. Results: Ten different gene cassette arrays were identified in 30 strains of E. coli. Cassettes corresponding to genes coding for adenylyltransferases (aadA), dihydrofolate reductases (dfrA), and oxacillinases (blaOXA) were detected. Array dfrA17-aadA5 was predominantly prevalent over the other arrays identified. Phylogenetic group A was the most predominant, followed by group B2 and D. Conclusions: This study demonstrates the presence of E. coli of vaginal origin carrying class 1 integrons, which are main genetic elements of capture of resistance genes, with the possibility of capturing new resistance cassettes. These evidences should serve for the modification of protocols in the diagnosis and treatment of aerobic vaginitis, and the development of policies for the rational use of antimicrobials.
KW - Antimicrobial resistance
KW - Class 1 integrons
KW - Escherichia coli
UR - http://www.scopus.com/inward/record.url?scp=85079155940&partnerID=8YFLogxK
U2 - 10.3855/jidc.11940
DO - 10.3855/jidc.11940
M3 - Artículo
C2 - 32088684
AN - SCOPUS:85079155940
SN - 2036-6590
VL - 14
SP - 48
EP - 58
JO - Journal of Infection in Developing Countries
JF - Journal of Infection in Developing Countries
IS - 1
ER -