TY - JOUR
T1 - Chromatographic analysis of bioactive proanthocyanidins from Quercus durifolia and Quercus eduardii barks
AU - Rosales-Castro, M.
AU - González-Laredo, R.
AU - Rocha-Guzmán, N.
AU - Gallegos-Infante, J.
AU - Peralta-Cruz, J.
AU - Morré, J.
AU - Karchesy, J.
PY - 2011/9/1
Y1 - 2011/9/1
N2 - Crude acetone extracts (CE) from Quercus durifolia and Quercus eduardii barks were partially purified by liquid extraction with ethyl acetate into organic extracts (OE), and these were separated by a Toyopearl HW-40F column chromatography with acetone-water (3:2) into oligomeric fractions (OLF). OEs were analyzed by HPLC and OLF by HPLC-MS. Screening of the antioxidant capacity of CE, OE, and OLF were performed by scavenging 1,1′-diphenyl-2- picrylhydrazyl (DPPH) radical and ABTS•+ radical cations as well by β-carotene-linoleic acid model system assays. In the bark of both Quercus species the major compound identified in OE was catechin. In OLF the major compounds were monomeric and dimeric monogallate procyanidins, and monomeric, dimeric, trimeric, and tetrameric procyanidins. The best antioxidant capacity was shown by OLF from bark extracts of both species.
AB - Crude acetone extracts (CE) from Quercus durifolia and Quercus eduardii barks were partially purified by liquid extraction with ethyl acetate into organic extracts (OE), and these were separated by a Toyopearl HW-40F column chromatography with acetone-water (3:2) into oligomeric fractions (OLF). OEs were analyzed by HPLC and OLF by HPLC-MS. Screening of the antioxidant capacity of CE, OE, and OLF were performed by scavenging 1,1′-diphenyl-2- picrylhydrazyl (DPPH) radical and ABTS•+ radical cations as well by β-carotene-linoleic acid model system assays. In the bark of both Quercus species the major compound identified in OE was catechin. In OLF the major compounds were monomeric and dimeric monogallate procyanidins, and monomeric, dimeric, trimeric, and tetrameric procyanidins. The best antioxidant capacity was shown by OLF from bark extracts of both species.
KW - ABTS
KW - DPPH
KW - Quercus
KW - antioxidant
KW - bark
KW - procyanidins
UR - http://www.scopus.com/inward/record.url?scp=80052681813&partnerID=8YFLogxK
U2 - 10.1556/AChrom.23.2011.3.12
DO - 10.1556/AChrom.23.2011.3.12
M3 - Artículo
SN - 1233-2356
VL - 23
SP - 521
EP - 529
JO - Acta Chromatographica
JF - Acta Chromatographica
IS - 3
ER -