Resumen
Rice (Oryza sativa L.) is economically important worldwide since it is the cereal most highly consumed by humans; so, it is important to improve its nutritional properties. This paper reports the results of the biochemical characterization of storage proteins in seeds of segregating generations (F2 and F4) from (Morelos A-92) [MA92; Indica] x Kosihikari [Kosi; japonica] and Kosi x MA92) rice hybrids as well as induction of androgenesis. The highest total protein content (10.77%) was detected in Kosi x MA92 (F2). The F4 generation from Kosi x MA92 genotype showed the highest levels of albumin, globulins, and prolamins as well as the lowest percentage of glutelins (21.48%). The electrophoretic profile of globulins isolated from Kosi x MA92 hybrid (segregant-F4) showed 13 bands with 13-110 kDa molecular weights while glutelins just three bands (13, 16, and 21 kDa). Androgenesis was induced in the N6 culture medium, supplemented with ANA, 2.0; KIN, 1.0; glycine, 2.0 and glutamine, 500 mg/L, maltose 5%, and agar 8 g/L. The conversion of embryogenic structures into plants was performed in Murashige & Skoog (MS) supplemented with AIA, 0.5; ANA, 0.5, and KIN, 2.0 mg/L, sucrose, 30 and agar, 8 g/L.
Título traducido de la contribución | Characterization of storage proteins and anther culture for the development of high nutritional quality rice genotypes |
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Idioma original | Español |
Páginas (desde-hasta) | 37-44 |
Número de páginas | 8 |
Publicación | Biotecnologia Aplicada |
Volumen | 22 |
N.º | 1 |
Estado | Publicada - 2005 |
Palabras clave
- Androgenesis
- Genetic improvement
- Hybrids
- Oryza sativa L.
- Storage proteins