TY - JOUR
T1 - Characterization of IS1389, a new member of the IS3 family of insertion sequences isolated from Xanthomonas campestris pv. amaranthicola
AU - Gómez, Pilar
AU - Ribas-Aparicio, Rosa M.
AU - Pélaez, Ana I.
AU - Rodicio, M. Rosario
N1 - Funding Information:
Acknowledgements P. Gómez was the recipient of a grant from the FYCIT (Asturias, Spain), and R. M. Ribas-Aparicio was the recipient of a fellowship from the COFAA-IPN and the Banco de México (Mexico). This work was supported by grant no. BIO91-0563 (CICYT; Spain).
PY - 1999
Y1 - 1999
N2 - IS1389, a new insertion sequence belonging to the IS3 family, has been identified in Xanthomonas campestris pv. amaranthicola. The genome of this bacterium contains at least 11 copies of the element, whereas no hybridizing sequences were detected in other Xanthomonas species [X. axonopodis, X. fragaridae, X. phaseoli, and X. (Stenotrophomonas) maltophila]. Two nearly identical copies of the element (IS1389-A and IS1389-B) were characterized. According to analysis of sequence alignments and similar structural features, IS1389 belongs to the IS407 subgroup of the IS3 family, which duplicates 4bp of target DNA upon insertion. IS1389-A was found in the proximity of the modification gene of the XamI restriction-modification system.
AB - IS1389, a new insertion sequence belonging to the IS3 family, has been identified in Xanthomonas campestris pv. amaranthicola. The genome of this bacterium contains at least 11 copies of the element, whereas no hybridizing sequences were detected in other Xanthomonas species [X. axonopodis, X. fragaridae, X. phaseoli, and X. (Stenotrophomonas) maltophila]. Two nearly identical copies of the element (IS1389-A and IS1389-B) were characterized. According to analysis of sequence alignments and similar structural features, IS1389 belongs to the IS407 subgroup of the IS3 family, which duplicates 4bp of target DNA upon insertion. IS1389-A was found in the proximity of the modification gene of the XamI restriction-modification system.
KW - IS1389
KW - IS3 family
KW - Insertion sequence
KW - XamI restriction-modification system
KW - Xanthomonas campestris pv. amaranthicola
UR - http://www.scopus.com/inward/record.url?scp=0345517298&partnerID=8YFLogxK
U2 - 10.1007/s002030050734
DO - 10.1007/s002030050734
M3 - Artículo
SN - 0302-8933
VL - 172
SP - 15
EP - 21
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 1
ER -