TY - JOUR
T1 - Characterization of functionalized multiwalled carbon nanotubes for use in an enzymatic sensor
AU - Guadarrama-Fernández, Leonor
AU - Chanona-Pérez, Jorge
AU - Manzo-Robledo, Arturo
AU - Calderón-Domínguez, Georgina
AU - Martínez-Rivas, Adrián
AU - Ortiz-López, Jaime
AU - Vargas-García, Jorge Roberto
N1 - Publisher Copyright:
© Microscopy Society of America 2014.
PY - 2014/4/11
Y1 - 2014/4/11
N2 - Carbon nanotubes (CNT) have proven to be materials with great potential for the construction of biosensors. Development of fast, simple, and low cost biosensors to follow reactions in bioprocesses, or to detect food contaminants such as toxins, chemical compounds, and microorganisms, is presently an important research topic. This report includes microscopy and spectroscopy to characterize raw and chemically modified multiwall carbon nanotubes (MWCNTs) synthesized by chemical vapor deposition with the intention of using them as the active transducer in bioprocessing sensors. MWCNT were simultaneously purified and functionalized by an acid mixture involving HNO3-H2SO4 and amyloglucosidase attached onto the chemically modified MWCNT surface. A 49.0% decrease in its enzymatic activity was observed. Raw, purified, and enzyme-modified MWCNTs were analyzed by scanning and transmission electron microscopy and Raman and X-ray photoelectron spectroscopy. These studies confirmed purification and functionalization of the CNTs. Finally, cyclic voltammetry electrochemistry was used for electrical characterization of CNTs, which showed promising results that can be useful for construction of electrochemical biosensors applied to biological areas.
AB - Carbon nanotubes (CNT) have proven to be materials with great potential for the construction of biosensors. Development of fast, simple, and low cost biosensors to follow reactions in bioprocesses, or to detect food contaminants such as toxins, chemical compounds, and microorganisms, is presently an important research topic. This report includes microscopy and spectroscopy to characterize raw and chemically modified multiwall carbon nanotubes (MWCNTs) synthesized by chemical vapor deposition with the intention of using them as the active transducer in bioprocessing sensors. MWCNT were simultaneously purified and functionalized by an acid mixture involving HNO3-H2SO4 and amyloglucosidase attached onto the chemically modified MWCNT surface. A 49.0% decrease in its enzymatic activity was observed. Raw, purified, and enzyme-modified MWCNTs were analyzed by scanning and transmission electron microscopy and Raman and X-ray photoelectron spectroscopy. These studies confirmed purification and functionalization of the CNTs. Finally, cyclic voltammetry electrochemistry was used for electrical characterization of CNTs, which showed promising results that can be useful for construction of electrochemical biosensors applied to biological areas.
KW - Raman spectroscopy
KW - XPS
KW - biosensor
KW - electrochemistry
KW - electron microscopy
KW - functionalized nanotubes
UR - http://www.scopus.com/inward/record.url?scp=84909997293&partnerID=8YFLogxK
U2 - 10.1017/S143192761401304X
DO - 10.1017/S143192761401304X
M3 - Artículo
SN - 1431-9276
VL - 20
SP - 1479
EP - 1485
JO - Microscopy and Microanalysis
JF - Microscopy and Microanalysis
IS - 5
ER -