TY - JOUR
T1 - Characterization of chaperonin 10 (Cpn10) from the intestinal human pathogen Entamoeba histolytica
AU - van der Giezen, Mark
AU - León-Avila, Gloria
AU - Tovar, Jorge
PY - 2005/9
Y1 - 2005/9
N2 - Entamoeba histolytica is the causative agent of amoebiasis, a poverty-related disease that kills an estimated 100 000 people each year. E histolytica does not contain 'standard mitochondria', but harbours mitochondrial remnant organelles called mitosomes. These organelles are characterized by the presence of mitochondrial chaperonin Cpn60, but little else is known about the functions and molecular composition of mitosomes. In this study, a gene encoding molecular chaperonin Cpn10 - the functional partner of Cpn60 - was cloned, and its structure and expression were characterized, as well as the cellular localization of its encoded protein. The 5′ untranslated region of the gene contains all of the structural promoter elements required for transcription in this organism. The amoebic Cpn10, like Cpn60, is not significantly upregulated upon heat-shock treatment. Computer-assisted protein modelling, and specific antibodies against Cpn10 and Cpn60, suggest that both proteins interact with each other, and that they function in the same intracellular compartment. Thus, E. histolytica appears to have retained at least two of the key molecular components required for the refolding of imported mitosomal proteins.
AB - Entamoeba histolytica is the causative agent of amoebiasis, a poverty-related disease that kills an estimated 100 000 people each year. E histolytica does not contain 'standard mitochondria', but harbours mitochondrial remnant organelles called mitosomes. These organelles are characterized by the presence of mitochondrial chaperonin Cpn60, but little else is known about the functions and molecular composition of mitosomes. In this study, a gene encoding molecular chaperonin Cpn10 - the functional partner of Cpn60 - was cloned, and its structure and expression were characterized, as well as the cellular localization of its encoded protein. The 5′ untranslated region of the gene contains all of the structural promoter elements required for transcription in this organism. The amoebic Cpn10, like Cpn60, is not significantly upregulated upon heat-shock treatment. Computer-assisted protein modelling, and specific antibodies against Cpn10 and Cpn60, suggest that both proteins interact with each other, and that they function in the same intracellular compartment. Thus, E. histolytica appears to have retained at least two of the key molecular components required for the refolding of imported mitosomal proteins.
UR - http://www.scopus.com/inward/record.url?scp=24944464260&partnerID=8YFLogxK
U2 - 10.1099/mic.0.28068-0
DO - 10.1099/mic.0.28068-0
M3 - Artículo
C2 - 16151221
AN - SCOPUS:24944464260
SN - 1350-0872
VL - 151
SP - 3107
EP - 3115
JO - Microbiology
JF - Microbiology
IS - 9
ER -